TY - JOUR
T1 - Mononuclear Co(III) and Ni(II) complexes with polypyridyl ligands, [Co(phen)2(taptp)]3+ and [Ni(phen)2(taptp)] 2+
T2 - Synthesis, Photocleavage and DNA-binding
AU - Morshedi, Mahbod
AU - Hadadzadeh, Hassan
PY - 2013/3
Y1 - 2013/3
N2 - Two novel, mixed ligand complexes of cobalt(III) and nickel(II), [Co(phen)2(taptp)]3+ (1) and [Ni(phen)2(taptp)] 2+ (2) (phen = 1,10-phenanthroline and taptp = 4,5,9,18- tetraazaphenanthreno [9,10-b]triphenylene), were synthesized and characterized by elemental analyses, UV-visible and NMR spectroscopies. The binding interactions of the two complexes with DNA have been investigated using absorption and emission spectroscopy methods and electrophoresis measurement mode. The intrinsic binding constants for these complexes to DNA are in the order of 105. In Tris buffer, the Co(III) complex shows a moderate luminescence which was enhanced after binding to DNA. However for complex Ni(II), no emission was observed in Tris buffer. The [Co(phen) 2(taptp)]3+ and [Ni(phen)2(taptp)]2+ can cause the photocleavage of DNA supercoiled pBR322 upon irradiation by 360 nm light. Based on the data, an intercalative mode of DNA binding is suggested for the two complexes.
AB - Two novel, mixed ligand complexes of cobalt(III) and nickel(II), [Co(phen)2(taptp)]3+ (1) and [Ni(phen)2(taptp)] 2+ (2) (phen = 1,10-phenanthroline and taptp = 4,5,9,18- tetraazaphenanthreno [9,10-b]triphenylene), were synthesized and characterized by elemental analyses, UV-visible and NMR spectroscopies. The binding interactions of the two complexes with DNA have been investigated using absorption and emission spectroscopy methods and electrophoresis measurement mode. The intrinsic binding constants for these complexes to DNA are in the order of 105. In Tris buffer, the Co(III) complex shows a moderate luminescence which was enhanced after binding to DNA. However for complex Ni(II), no emission was observed in Tris buffer. The [Co(phen) 2(taptp)]3+ and [Ni(phen)2(taptp)]2+ can cause the photocleavage of DNA supercoiled pBR322 upon irradiation by 360 nm light. Based on the data, an intercalative mode of DNA binding is suggested for the two complexes.
KW - Co (III) and Ni(II) complexes
KW - DNA binding
KW - Intercalative mode
KW - Mixed-ligand
KW - Photocleavage
KW - Polypyridyl ligands
UR - http://www.scopus.com/inward/record.url?scp=84879686120&partnerID=8YFLogxK
U2 - 10.1007/s10895-012-1143-0
DO - 10.1007/s10895-012-1143-0
M3 - Article
SN - 1053-0509
VL - 23
SP - 259
EP - 264
JO - Journal of Fluorescence
JF - Journal of Fluorescence
IS - 2
ER -