@inproceedings{08c5dcc1ae054d238628007126061a0f,
title = "Multi-site optical recording of neuronal activity with complex light patterns",
abstract = "We use complex light patterns to simultaneously record the neuronal activity along the dendrites of a single neuron. We use holographic projection to produce multiple foci directed onto different dendritic regions of the neuron. Each focus excites neuronal activity reporters via either two-photon (2P) or single-photon (1P) excitation. The fluorescence emanating from all foci are simultaneously recorded using an electron-multiplying charge-coupled device (EMCCD) camera thereby enabling simultaneous multi-channel recording of the neuronal activity from multiple sites at high frame rates (up to 400Hz). We report recording of neuronal activity from two types of reporters: (1) Ca2+ indicator, Cal-520; and (2) voltage indicator, JPW-1114. We optically recorded the activity evoked by the neuron following injection of current onto the soma. Holographic multi-site Ca2+ imaging resulted in high signal-to-noise ratio but with poor temporal resolution. On the other hand, multi-site voltage imaging produced noisy and low SNR signals but with high temporal resolution that is able to resolve action potentials.",
keywords = "Holographic projection, Two-photon functional calcium imaging, Two-photon imaging, Voltage imaging",
author = "Casta{\~n}ares, {Michael Lawrence} and Daria, {Vincent R.}",
note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; Complex Light and Optical Forces XIII 2019 ; Conference date: 05-02-2019 Through 07-02-2019",
year = "2019",
doi = "10.1117/12.2511683",
language = "English",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Galvez, {Enrique J.} and Andrews, {David L.} and Jesper Gluckstad",
booktitle = "Complex Light and Optical Forces XIII",
address = "United States",
}