TY - JOUR
T1 - N-glycan trimming by glucosidase II is essential for Arabidopsis development
AU - Soussillane, Pravina
AU - D'Alessio, Cecilia
AU - Paccalet, Thomas
AU - Fitchette, Anne Catherine
AU - Parodi, Armando J.
AU - Williamson, Richard
AU - Plasson, Carole
AU - Faye, Loïc
AU - Gomord, Véronique
PY - 2009/7
Y1 - 2009/7
N2 - Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble heterodimer composed of α and β subunits. Here we present the first characterization of a plant glucosidase II α subunit at the molecular level. Expression of the Arabidopsis α subunit restored N-glycan maturation capacity in Schizosaccharomyces pombe α- or αβ-deficient mutants, but with a lower efficiency in the last case. Inactivation of the α subunit in a temperature sensitive Arabidopsis mutant blocked N-glycan processing after a first trimming by glucosidase I and strongly affected seedling development.
AB - Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble heterodimer composed of α and β subunits. Here we present the first characterization of a plant glucosidase II α subunit at the molecular level. Expression of the Arabidopsis α subunit restored N-glycan maturation capacity in Schizosaccharomyces pombe α- or αβ-deficient mutants, but with a lower efficiency in the last case. Inactivation of the α subunit in a temperature sensitive Arabidopsis mutant blocked N-glycan processing after a first trimming by glucosidase I and strongly affected seedling development.
KW - Glucose-trimming
KW - Glucosidase II
KW - N-glycosylation
KW - Plant
UR - http://www.scopus.com/inward/record.url?scp=68949127198&partnerID=8YFLogxK
U2 - 10.1007/s10719-008-9201-1
DO - 10.1007/s10719-008-9201-1
M3 - Article
SN - 0282-0080
VL - 26
SP - 597
EP - 607
JO - Glycoconjugate Journal
JF - Glycoconjugate Journal
IS - 5
ER -