Nanostructure of the protein-nanoparticle corona an indicator of toxicity?

John W. White*, Jhih Min Lin, Joo Chuan Ang, Richard A. Campbell, Valerie Laux, Michael Haertlein, Giovanna Fragneto

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    Nanoparticle-protein complexes comprising silica or cadmium sulfide nanoparticles with human proteins have been detected with high sensitivity at the air-water interface using X-ray and neutron reflectivity measurements and ellipsometry. For the interaction between-casein and 8 nm silica particles the sensitivity of the reflectivity signal at the air-water interface has been shown by X-ray reflectmetry to be pico-molar. This high sensitivity results from the nanoparticle-protein interaction which generates surface active complexes. The interaction between human serum albumin and cadmium sulfide nanoparticles has been detected using neutron reflectometry and tracked kinetically by ellipsometry. Here we have shown that lateral domains or aggregates in the surface monolayer change in size or dissipate over the course of several hours. The three methods of monitoring the protein-nanoparticle interaction during film evolution are discussed.

    Original languageEnglish
    Title of host publicationICONN 2010 - Proceedings of the 2010 International Conference on Nanoscience and Nanotechnology
    Pages289-292
    Number of pages4
    DOIs
    Publication statusPublished - 2010
    Event2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN 2010 - Sydney, NSW, Australia
    Duration: 22 Feb 201026 Feb 2010

    Publication series

    NameICONN 2010 - Proceedings of the 2010 International Conference on Nanoscience and Nanotechnology

    Conference

    Conference2010 3rd International Conference on Nanoscience and Nanotechnology, ICONN 2010
    Country/TerritoryAustralia
    CitySydney, NSW
    Period22/02/1026/02/10

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