New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad species (Bufo viridis subgroup)

Caroline Colliard; Alessandra Sicilia; Craig Moritz; Nicolas Perrin; Matthias Stöck

doi:10.1111/j.1755-0998.2009.02600.x

New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad species (Bufo viridis subgroup)

Caroline Colliard, Alessandra Sicilia, Craig Moritz, Nicolas Perrin, Matthias Stöck^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We report new polymorphic microsatellites for three species of Palearctic green toads (Bufo viridis subgroup): 10 in B. balearicus and seven each in B. siculus and B. boulengeri. Diversity at these loci, measured for 27 B. balearicus, 23 B. siculus and 11 B. boulengeri, ranged from low to high (two to 10 alleles). Mitotyping primers, specific to the control region, which allow fast screening of parapatric Sicilian endemic B. siculus and Italian mainland-origin B. balearicus, were developed.

Original language	English
Pages (from-to)	1138-1140
Number of pages	3
Journal	Molecular Ecology Resources
Volume	9
Issue number	4
DOIs	https://doi.org/10.1111/j.1755-0998.2009.02600.x
Publication status	Published - Jul 2009
Externally published	Yes

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10.1111/j.1755-0998.2009.02600.x

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title = "New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad species (Bufo viridis subgroup)",

abstract = "We report new polymorphic microsatellites for three species of Palearctic green toads (Bufo viridis subgroup): 10 in B. balearicus and seven each in B. siculus and B. boulengeri. Diversity at these loci, measured for 27 B. balearicus, 23 B. siculus and 11 B. boulengeri, ranged from low to high (two to 10 alleles). Mitotyping primers, specific to the control region, which allow fast screening of parapatric Sicilian endemic B. siculus and Italian mainland-origin B. balearicus, were developed.",

keywords = "Bufo viridis subgroup, Cross-priming, Microsatellites, Mitotyping, Palearctic green toads",

author = "Caroline Colliard and Alessandra Sicilia and Craig Moritz and Nicolas Perrin and Matthias St{\"o}ck",

year = "2009",

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T1 - New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad species (Bufo viridis subgroup)

AU - Colliard, Caroline

AU - Sicilia, Alessandra

AU - Moritz, Craig

AU - Perrin, Nicolas

AU - Stöck, Matthias

PY - 2009/7

Y1 - 2009/7

N2 - We report new polymorphic microsatellites for three species of Palearctic green toads (Bufo viridis subgroup): 10 in B. balearicus and seven each in B. siculus and B. boulengeri. Diversity at these loci, measured for 27 B. balearicus, 23 B. siculus and 11 B. boulengeri, ranged from low to high (two to 10 alleles). Mitotyping primers, specific to the control region, which allow fast screening of parapatric Sicilian endemic B. siculus and Italian mainland-origin B. balearicus, were developed.

AB - We report new polymorphic microsatellites for three species of Palearctic green toads (Bufo viridis subgroup): 10 in B. balearicus and seven each in B. siculus and B. boulengeri. Diversity at these loci, measured for 27 B. balearicus, 23 B. siculus and 11 B. boulengeri, ranged from low to high (two to 10 alleles). Mitotyping primers, specific to the control region, which allow fast screening of parapatric Sicilian endemic B. siculus and Italian mainland-origin B. balearicus, were developed.

KW - Bufo viridis subgroup

KW - Cross-priming

KW - Microsatellites

KW - Mitotyping

KW - Palearctic green toads

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U2 - 10.1111/j.1755-0998.2009.02600.x

DO - 10.1111/j.1755-0998.2009.02600.x

M3 - Article

SN - 1755-098X

VL - 9

SP - 1138

EP - 1140

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

IS - 4

ER -

New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad species (Bufo viridis subgroup)

Abstract

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