Next-generation genome editing: no transgene, no tissue culture

Muhammad Arslan Mahmood; Julian R. Greenwood; Anthony A. Millar; Hendry Susila

doi:10.1016/j.tplants.2025.12.008

Next-generation genome editing: no transgene, no tissue culture

Muhammad Arslan Mahmood
, Julian R. Greenwood
, Anthony A. Millar
, Hendry Susila^*

^*Corresponding author for this work

Research output: Contribution to journal › Short survey › peer-review

3 Citations (Scopus)

Abstract

New approaches to engineering plant genomes have the potential to improve agriculture. However, transgenes insertion and tissue culture have become bottlenecks to genome-editing technology becoming widely adopted and achieving the promise of targeted editing. Recent developments in particle bombardment and viral vector-mediated delivery can open doors to overcome these limitations.

Original language	English
Pages (from-to)	383-385
Number of pages	3
Journal	Trends in Plant Science
Volume	31
Issue number	4
DOIs	https://doi.org/10.1016/j.tplants.2025.12.008
Publication status	Published - Apr 2026

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10.1016/j.tplants.2025.12.008

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title = "Next-generation genome editing: no transgene, no tissue culture",

abstract = "New approaches to engineering plant genomes have the potential to improve agriculture. However, transgenes insertion and tissue culture have become bottlenecks to genome-editing technology becoming widely adopted and achieving the promise of targeted editing. Recent developments in particle bombardment and viral vector-mediated delivery can open doors to overcome these limitations.",

keywords = "genome editing, tissue culture-free, transgene-free",

author = "Mahmood, \{Muhammad Arslan\} and Greenwood, \{Julian R.\} and Millar, \{Anthony A.\} and Hendry Susila",

note = "Publisher Copyright: {\textcopyright} 2025 Elsevier Ltd.",

year = "2026",

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doi = "10.1016/j.tplants.2025.12.008",

language = "English",

volume = "31",

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journal = "Trends in Plant Science",

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T1 - Next-generation genome editing

T2 - no transgene, no tissue culture

AU - Mahmood, Muhammad Arslan

AU - Greenwood, Julian R.

AU - Millar, Anthony A.

AU - Susila, Hendry

PY - 2026/4

Y1 - 2026/4

N2 - New approaches to engineering plant genomes have the potential to improve agriculture. However, transgenes insertion and tissue culture have become bottlenecks to genome-editing technology becoming widely adopted and achieving the promise of targeted editing. Recent developments in particle bombardment and viral vector-mediated delivery can open doors to overcome these limitations.

AB - New approaches to engineering plant genomes have the potential to improve agriculture. However, transgenes insertion and tissue culture have become bottlenecks to genome-editing technology becoming widely adopted and achieving the promise of targeted editing. Recent developments in particle bombardment and viral vector-mediated delivery can open doors to overcome these limitations.

KW - genome editing

KW - tissue culture-free

KW - transgene-free

UR - https://www.scopus.com/pages/publications/105026463300

U2 - 10.1016/j.tplants.2025.12.008

DO - 10.1016/j.tplants.2025.12.008

M3 - Short survey

C2 - 41494883

AN - SCOPUS:105026463300

SN - 1360-1385

VL - 31

SP - 383

EP - 385

JO - Trends in Plant Science

JF - Trends in Plant Science

IS - 4

ER -

Next-generation genome editing: no transgene, no tissue culture

Abstract

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