NMDA receptors at excitatory synapses in the hippocampus: test of a theory of magnesium block

John M. Bekkers; Charles F. Stevens

doi:10.1016/0304-3940(93)90443-O

NMDA receptors at excitatory synapses in the hippocampus: test of a theory of magnesium block

John M. Bekkers^*, Charles F. Stevens

^*Corresponding author for this work

The Australian National University

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Excitatory postsynaptic currents (EPSCs) were studied using the whole-cell patch-clamp technique to record from rat hippocampal pyramidal neurons in both slices and dissociated cultures. The voltage- and magnesium-dependence of the N-methyl-d-aspartate (NMDA) component of the EPSCs was quantified by measuring the amplitude of this component at a range of holding potentials (-80 mV to 60 or 80 mV) in several bath magnesium concentrations (0.2μM, 0.1, 1 and 10 mM). All of our results were well-fitted by a theory of magnesium block developed from single-channel studies of extrajunctional NMDA channels in culture. It is concluded that extrasynaptic and subsynaptic NMDA channels both in culture and in situ have identical properties of magnesium block.

Original language	English
Pages (from-to)	73-77
Number of pages	5
Journal	Neuroscience Letters
Volume	156
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(93)90443-O
Publication status	Published - 25 Jun 1993

Access to Document

10.1016/0304-3940(93)90443-O

Cite this

@article{b54a56cbc3a345febe20d1ea13684baa,

title = "NMDA receptors at excitatory synapses in the hippocampus: test of a theory of magnesium block",

abstract = "Excitatory postsynaptic currents (EPSCs) were studied using the whole-cell patch-clamp technique to record from rat hippocampal pyramidal neurons in both slices and dissociated cultures. The voltage- and magnesium-dependence of the N-methyl-d-aspartate (NMDA) component of the EPSCs was quantified by measuring the amplitude of this component at a range of holding potentials (-80 mV to 60 or 80 mV) in several bath magnesium concentrations (0.2μM, 0.1, 1 and 10 mM). All of our results were well-fitted by a theory of magnesium block developed from single-channel studies of extrajunctional NMDA channels in culture. It is concluded that extrasynaptic and subsynaptic NMDA channels both in culture and in situ have identical properties of magnesium block.",

keywords = "EPSC, Hippocampus, Long-term potentiation, Magnesium, N-Methyl-d-aspartate, Voltage dependence",

author = "Bekkers, {John M.} and Stevens, {Charles F.}",

year = "1993",

month = jun,

day = "25",

doi = "10.1016/0304-3940(93)90443-O",

language = "English",

volume = "156",

pages = "73--77",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

}

TY - JOUR

T1 - NMDA receptors at excitatory synapses in the hippocampus

T2 - test of a theory of magnesium block

AU - Bekkers, John M.

AU - Stevens, Charles F.

PY - 1993/6/25

Y1 - 1993/6/25

N2 - Excitatory postsynaptic currents (EPSCs) were studied using the whole-cell patch-clamp technique to record from rat hippocampal pyramidal neurons in both slices and dissociated cultures. The voltage- and magnesium-dependence of the N-methyl-d-aspartate (NMDA) component of the EPSCs was quantified by measuring the amplitude of this component at a range of holding potentials (-80 mV to 60 or 80 mV) in several bath magnesium concentrations (0.2μM, 0.1, 1 and 10 mM). All of our results were well-fitted by a theory of magnesium block developed from single-channel studies of extrajunctional NMDA channels in culture. It is concluded that extrasynaptic and subsynaptic NMDA channels both in culture and in situ have identical properties of magnesium block.

AB - Excitatory postsynaptic currents (EPSCs) were studied using the whole-cell patch-clamp technique to record from rat hippocampal pyramidal neurons in both slices and dissociated cultures. The voltage- and magnesium-dependence of the N-methyl-d-aspartate (NMDA) component of the EPSCs was quantified by measuring the amplitude of this component at a range of holding potentials (-80 mV to 60 or 80 mV) in several bath magnesium concentrations (0.2μM, 0.1, 1 and 10 mM). All of our results were well-fitted by a theory of magnesium block developed from single-channel studies of extrajunctional NMDA channels in culture. It is concluded that extrasynaptic and subsynaptic NMDA channels both in culture and in situ have identical properties of magnesium block.

KW - EPSC

KW - Hippocampus

KW - Long-term potentiation

KW - Magnesium

KW - N-Methyl-d-aspartate

KW - Voltage dependence

UR - http://www.scopus.com/inward/record.url?scp=0027283707&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(93)90443-O

DO - 10.1016/0304-3940(93)90443-O

M3 - Article

C2 - 8414193

AN - SCOPUS:0027283707

SN - 0304-3940

VL - 156

SP - 73

EP - 77

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

NMDA receptors at excitatory synapses in the hippocampus: test of a theory of magnesium block

Abstract

Access to Document

Other files and links

Fingerprint

Cite this