NMR structure determination of protein - Ligand complexes by lanthanide labeling

Guido Pintacuda; Michael John; Xun Cheng Su; Gottfried Otting

doi:10.1021/ar050087z

NMR structure determination of protein - Ligand complexes by lanthanide labeling

Guido Pintacuda, Michael John, Xun Cheng Su, Gottfried Otting^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

250 Citations (Scopus)

Abstract

The paramagnetism of lanthanide ions offers outstanding opportunities for fast determinations of the three-dimensional (3D) structures of protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy. It is shown how the combination of pseudocontact shifts (PCSs) induced by a site-specifically bound lanthanide ion and prior knowledge of the 3D structure of the lanthanide-labeled protein can be used to achieve (i) rapid assignments of NMR spectra, (ii) structure determinations of protein-protein complexes, and (iii) identification of the binding mode of low-molecular weight compounds in complexes with proteins. Strategies for site-specific incorporation of lanthanide ions into proteins are summarized.

Original language	English
Pages (from-to)	206-212
Number of pages	7
Journal	Accounts of Chemical Research
Volume	40
Issue number	3
DOIs	https://doi.org/10.1021/ar050087z
Publication status	Published - Mar 2007

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abstract = "The paramagnetism of lanthanide ions offers outstanding opportunities for fast determinations of the three-dimensional (3D) structures of protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy. It is shown how the combination of pseudocontact shifts (PCSs) induced by a site-specifically bound lanthanide ion and prior knowledge of the 3D structure of the lanthanide-labeled protein can be used to achieve (i) rapid assignments of NMR spectra, (ii) structure determinations of protein-protein complexes, and (iii) identification of the binding mode of low-molecular weight compounds in complexes with proteins. Strategies for site-specific incorporation of lanthanide ions into proteins are summarized.",

author = "Guido Pintacuda and Michael John and Su, {Xun Cheng} and Gottfried Otting",

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journal = "Accounts of Chemical Research",

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T1 - NMR structure determination of protein - Ligand complexes by lanthanide labeling

AU - Pintacuda, Guido

AU - John, Michael

AU - Su, Xun Cheng

AU - Otting, Gottfried

PY - 2007/3

Y1 - 2007/3

N2 - The paramagnetism of lanthanide ions offers outstanding opportunities for fast determinations of the three-dimensional (3D) structures of protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy. It is shown how the combination of pseudocontact shifts (PCSs) induced by a site-specifically bound lanthanide ion and prior knowledge of the 3D structure of the lanthanide-labeled protein can be used to achieve (i) rapid assignments of NMR spectra, (ii) structure determinations of protein-protein complexes, and (iii) identification of the binding mode of low-molecular weight compounds in complexes with proteins. Strategies for site-specific incorporation of lanthanide ions into proteins are summarized.

AB - The paramagnetism of lanthanide ions offers outstanding opportunities for fast determinations of the three-dimensional (3D) structures of protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy. It is shown how the combination of pseudocontact shifts (PCSs) induced by a site-specifically bound lanthanide ion and prior knowledge of the 3D structure of the lanthanide-labeled protein can be used to achieve (i) rapid assignments of NMR spectra, (ii) structure determinations of protein-protein complexes, and (iii) identification of the binding mode of low-molecular weight compounds in complexes with proteins. Strategies for site-specific incorporation of lanthanide ions into proteins are summarized.

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U2 - 10.1021/ar050087z

DO - 10.1021/ar050087z

M3 - Article

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VL - 40

SP - 206

EP - 212

JO - Accounts of Chemical Research

JF - Accounts of Chemical Research

IS - 3

ER -

NMR structure determination of protein - Ligand complexes by lanthanide labeling

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