Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis

Y. T.Candace Ho, Joe A. Kaczmarski, Julien Tailhades, Thierry Izoré, David L. Steer, Ralf B. Schittenhelm, Manuela Tosin, Colin J. Jackson, Max J. Cryle*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    Nonribosomal peptide synthetases produce many important peptide natural products and are centred around carrier proteins (CPs) that deliver intermediates to various catalytic domains. We show that the replacement of CP substrate thioesters by stabilised ester analogues leads to active condensation domain complexes, whereas amide stabilisation generates non-functional complexes.

    Original languageEnglish
    Pages (from-to)8234-8237
    Number of pages4
    JournalChemical Communications
    Volume59
    DOIs
    Publication statusPublished - 8 Jun 2023

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