TY - JOUR
T1 - Novel approaches for identifying genes regulating lymphocyte development and function
AU - Loy, Adèle L.
AU - Goodnow, Christopher C.
PY - 2002/4/1
Y1 - 2002/4/1
N2 - The draft sequence of the human and mouse genomes provides an unparalleled opportunity for understanding the genetic control of immune-cell development. Strategies can begin with a gene sequence and pursue a putative immune-system function by employing mRNA-expression profiling or creating gene knockouts in embryonic stem cells. The latter can be produced by utilising the Cre/Lox system, a tetracycline operon, a gene-trap method or chemical mutagenesis. Alternatively, mutant phenotypes (derived using the mutagen ethylnitrosourea) can be traced back to gene sequences.
AB - The draft sequence of the human and mouse genomes provides an unparalleled opportunity for understanding the genetic control of immune-cell development. Strategies can begin with a gene sequence and pursue a putative immune-system function by employing mRNA-expression profiling or creating gene knockouts in embryonic stem cells. The latter can be produced by utilising the Cre/Lox system, a tetracycline operon, a gene-trap method or chemical mutagenesis. Alternatively, mutant phenotypes (derived using the mutagen ethylnitrosourea) can be traced back to gene sequences.
UR - http://www.scopus.com/inward/record.url?scp=0036533338&partnerID=8YFLogxK
U2 - 10.1016/S0952-7915(02)00331-X
DO - 10.1016/S0952-7915(02)00331-X
M3 - Review article
SN - 0952-7915
VL - 14
SP - 260
EP - 265
JO - Current Opinion in Immunology
JF - Current Opinion in Immunology
IS - 2
ER -