Novel microsatellite loci identified from the Australian eastern small-eyed snake (Elapidae: Rhinocephalus nigrescens) and cross species amplification in the related genus Suta

Jessica Stapley; Christine M. Hayes; Jonathan K. Webb; J. Scott Keogh

doi:10.1111/j.1471-8286.2004.00832.x

Novel microsatellite loci identified from the Australian eastern small-eyed snake (Elapidae: Rhinocephalus nigrescens) and cross species amplification in the related genus Suta

Jessica Stapley, Christine M. Hayes, Jonathan K. Webb, J. Scott Keogh^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A total of 15 microsatellite primers pairs were developed for the Australian small-eyed snake Rhinoplocephalus nigrescens. Five primers were used to screen 93 individuals of R. nigrescens and were also tested against eight species of the closely related genus Suta. Allelic diversity in R. nigrescens was high in three loci (12-27) and there was high heterozygosity (0.58-0.82). Observed heterozygosity did not deviate from Hardy-Weinberg expectations for the five loci tested. These primers will be useful in studies of population genetics and mating systems of small-eyed snakes and related species.

Original language	English
Pages (from-to)	54-56
Number of pages	3
Journal	Molecular Ecology Notes
Volume	5
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-8286.2004.00832.x
Publication status	Published - Mar 2005

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title = "Novel microsatellite loci identified from the Australian eastern small-eyed snake (Elapidae: Rhinocephalus nigrescens) and cross species amplification in the related genus Suta",

abstract = "A total of 15 microsatellite primers pairs were developed for the Australian small-eyed snake Rhinoplocephalus nigrescens. Five primers were used to screen 93 individuals of R. nigrescens and were also tested against eight species of the closely related genus Suta. Allelic diversity in R. nigrescens was high in three loci (12-27) and there was high heterozygosity (0.58-0.82). Observed heterozygosity did not deviate from Hardy-Weinberg expectations for the five loci tested. These primers will be useful in studies of population genetics and mating systems of small-eyed snakes and related species.",

keywords = "Australia, Elapidae, Microsatellite primers, Snake",

author = "Jessica Stapley and Hayes, {Christine M.} and Webb, {Jonathan K.} and Keogh, {J. Scott}",

year = "2005",

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doi = "10.1111/j.1471-8286.2004.00832.x",

language = "English",

volume = "5",

pages = "54--56",

journal = "Molecular Ecology Notes",

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Novel microsatellite loci identified from the Australian eastern small-eyed snake (Elapidae: Rhinocephalus nigrescens) and cross species amplification in the related genus Suta. / Stapley, Jessica; Hayes, Christine M.; Webb, Jonathan K. et al.
In: Molecular Ecology Notes, Vol. 5, No. 1, 03.2005, p. 54-56.

Research output: Contribution to journal › Article › peer-review

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T1 - Novel microsatellite loci identified from the Australian eastern small-eyed snake (Elapidae: Rhinocephalus nigrescens) and cross species amplification in the related genus Suta

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AU - Webb, Jonathan K.

AU - Keogh, J. Scott

PY - 2005/3

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AB - A total of 15 microsatellite primers pairs were developed for the Australian small-eyed snake Rhinoplocephalus nigrescens. Five primers were used to screen 93 individuals of R. nigrescens and were also tested against eight species of the closely related genus Suta. Allelic diversity in R. nigrescens was high in three loci (12-27) and there was high heterozygosity (0.58-0.82). Observed heterozygosity did not deviate from Hardy-Weinberg expectations for the five loci tested. These primers will be useful in studies of population genetics and mating systems of small-eyed snakes and related species.

KW - Australia

KW - Elapidae

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JO - Molecular Ecology Notes

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IS - 1

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Novel microsatellite loci identified from the Australian eastern small-eyed snake (Elapidae: Rhinocephalus nigrescens) and cross species amplification in the related genus Suta

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