Nucleic Acid Circular Dichroism

Alison Rodger

doi:10.1007/978-3-642-16712-6_632

Nucleic Acid Circular Dichroism

Alison Rodger^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Entry for encyclopedia/dictionary › peer-review

1 Citation (Scopus)

Abstract

Synonyms

DNA

Definition

Deoxyribonucleic acid, DNA, is composed of planar aromatic nucleic acid bases linked to a sugar and a phosphate as illustrated in Fig. 1. Ribonucleic acid, RNA, differs from DNA by an extra oxygen on the sugar and also thymine is replaced by uracil which does not have the methyl substituent. Most of the DNA and RNA transitions we measure with UV spectroscopy is due to the π–π* transitions of the bases. These transitions have no intrinsic CD signal since the bases are planar and hence achiral. However, the isolated bases acquire asymmetry in their electronic transitions by coupling with the chiral ribose sugar units of the backbone. The magnitude of Δε_max (the molar difference in absorbance of left and right circularly polarized light) for each base is of the order of 2 mol⁻¹ dm³ cm⁻¹at 270 nm; the purine bases have a negative signal whereas the pyrimidine ones have a positive CD in that region. When the bases are linked by phosphodiester bonds to form DNA or...

Original language	English
Title of host publication	Encyclopedia of Biophysics
Editors	Gordon C. K. Roberts
Place of Publication	Berlin, Heidelberg
Publisher	Springer Science+Business Media B.V.
Pages	1759-1761
Number of pages	3
ISBN (Electronic)	9783642167126
ISBN (Print)	9783642167119
DOIs	https://doi.org/10.1007/978-3-642-16712-6_632
Publication status	Published - 2013
Externally published	Yes

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10.1007/978-3-642-16712-6_632

Cite this

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title = "Nucleic Acid Circular Dichroism",

abstract = "SynonymsDNA DefinitionDeoxyribonucleic acid, DNA, is composed of planar aromatic nucleic acid bases linked to a sugar and a phosphate as illustrated in Fig. 1. Ribonucleic acid, RNA, differs from DNA by an extra oxygen on the sugar and also thymine is replaced by uracil which does not have the methyl substituent. Most of the DNA and RNA transitions we measure with UV spectroscopy is due to the π–π* transitions of the bases. These transitions have no intrinsic CD signal since the bases are planar and hence achiral. However, the isolated bases acquire asymmetry in their electronic transitions by coupling with the chiral ribose sugar units of the backbone. The magnitude of Δεmax (the molar difference in absorbance of left and right circularly polarized light) for each base is of the order of 2 mol−1 dm3 cm−1 at 270 nm; the purine bases have a negative signal whereas the pyrimidine ones have a positive CD in that region. When the bases are linked by phosphodiester bonds to form DNA or...",

author = "Alison Rodger",

year = "2013",

doi = "10.1007/978-3-642-16712-6\_632",

language = "English",

isbn = "9783642167119",

pages = "1759--1761",

editor = "Roberts, \{Gordon C. K.\}",

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PY - 2013

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N2 - SynonymsDNA DefinitionDeoxyribonucleic acid, DNA, is composed of planar aromatic nucleic acid bases linked to a sugar and a phosphate as illustrated in Fig. 1. Ribonucleic acid, RNA, differs from DNA by an extra oxygen on the sugar and also thymine is replaced by uracil which does not have the methyl substituent. Most of the DNA and RNA transitions we measure with UV spectroscopy is due to the π–π* transitions of the bases. These transitions have no intrinsic CD signal since the bases are planar and hence achiral. However, the isolated bases acquire asymmetry in their electronic transitions by coupling with the chiral ribose sugar units of the backbone. The magnitude of Δεmax (the molar difference in absorbance of left and right circularly polarized light) for each base is of the order of 2 mol−1 dm3 cm−1 at 270 nm; the purine bases have a negative signal whereas the pyrimidine ones have a positive CD in that region. When the bases are linked by phosphodiester bonds to form DNA or...

AB - SynonymsDNA DefinitionDeoxyribonucleic acid, DNA, is composed of planar aromatic nucleic acid bases linked to a sugar and a phosphate as illustrated in Fig. 1. Ribonucleic acid, RNA, differs from DNA by an extra oxygen on the sugar and also thymine is replaced by uracil which does not have the methyl substituent. Most of the DNA and RNA transitions we measure with UV spectroscopy is due to the π–π* transitions of the bases. These transitions have no intrinsic CD signal since the bases are planar and hence achiral. However, the isolated bases acquire asymmetry in their electronic transitions by coupling with the chiral ribose sugar units of the backbone. The magnitude of Δεmax (the molar difference in absorbance of left and right circularly polarized light) for each base is of the order of 2 mol−1 dm3 cm−1 at 270 nm; the purine bases have a negative signal whereas the pyrimidine ones have a positive CD in that region. When the bases are linked by phosphodiester bonds to form DNA or...

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ER -

Nucleic Acid Circular Dichroism

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