Nucleic Acid Linear Dichroism

Alison Rodger

doi:10.1007/978-3-642-16712-6_631

Nucleic Acid Linear Dichroism

Alison Rodger^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Entry for encyclopedia/dictionary › peer-review

Abstract

Synonyms
Anisotropic spectroscopy; DNA

Definition
A linear dichroism (LD) spectrum shows the difference in absorbance of light polarized parallel and perpendicular to an orientation direction (Rodger and Nordén 1997; Nordén et al 2010). The idealized structure of B-DNA has the base pairs oriented perpendicular to the DNA helix axis to a first approximation. This means the π–π* transitions take place in the plane perpendicular to the helix axis, and so these are expected to give a negative LD signal as illustrated in Fig. 1. LD can be used to probe changes in orientation of the DNA bases.

Original language	English
Title of host publication	Encyclopedia of Biophysics
Editors	Gordon C. K. Roberts
Place of Publication	Berlin, Heidelberg
Publisher	Springer Science+Business Media B.V.
Pages	1761-1763
Number of pages	3
ISBN (Electronic)	9783642167126
ISBN (Print)	9783642167119
DOIs	https://doi.org/10.1007/978-3-642-16712-6_631
Publication status	Published - 2013
Externally published	Yes

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10.1007/978-3-642-16712-6_631

Cite this

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title = "Nucleic Acid Linear Dichroism",

abstract = "Synonyms Anisotropic spectroscopy; DNA Definition A linear dichroism (LD) spectrum shows the difference in absorbance of light polarized parallel and perpendicular to an orientation direction (Rodger and Nord{\'e}n 1997; Nord{\'e}n et al 2010). The idealized structure of B-DNA has the base pairs oriented perpendicular to the DNA helix axis to a first approximation. This means the π–π* transitions take place in the plane perpendicular to the helix axis, and so these are expected to give a negative LD signal as illustrated in Fig. 1. LD can be used to probe changes in orientation of the DNA bases.",

author = "Alison Rodger",

year = "2013",

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T1 - Nucleic Acid Linear Dichroism

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PY - 2013

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N2 - Synonyms Anisotropic spectroscopy; DNA Definition A linear dichroism (LD) spectrum shows the difference in absorbance of light polarized parallel and perpendicular to an orientation direction (Rodger and Nordén 1997; Nordén et al 2010). The idealized structure of B-DNA has the base pairs oriented perpendicular to the DNA helix axis to a first approximation. This means the π–π* transitions take place in the plane perpendicular to the helix axis, and so these are expected to give a negative LD signal as illustrated in Fig. 1. LD can be used to probe changes in orientation of the DNA bases.

AB - Synonyms Anisotropic spectroscopy; DNA Definition A linear dichroism (LD) spectrum shows the difference in absorbance of light polarized parallel and perpendicular to an orientation direction (Rodger and Nordén 1997; Nordén et al 2010). The idealized structure of B-DNA has the base pairs oriented perpendicular to the DNA helix axis to a first approximation. This means the π–π* transitions take place in the plane perpendicular to the helix axis, and so these are expected to give a negative LD signal as illustrated in Fig. 1. LD can be used to probe changes in orientation of the DNA bases.

UR - https://www.scopus.com/pages/publications/105011798429

U2 - 10.1007/978-3-642-16712-6_631

DO - 10.1007/978-3-642-16712-6_631

M3 - Entry for encyclopedia/dictionary

AN - SCOPUS:105011798429

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BT - Encyclopedia of Biophysics

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PB - Springer Science+Business Media B.V.

CY - Berlin, Heidelberg

ER -

Nucleic Acid Linear Dichroism

Abstract

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