Nucleotide-level profiling of M5C RNA methylation

Tennille Sibbritt; Andrew Shafik; Susan J. Clark; Thomas Preiss

doi:10.1007/978-1-4939-3067-8_16

Nucleotide-level profiling of M⁵C RNA methylation

Tennille Sibbritt, Andrew Shafik, Susan J. Clark, Thomas Preiss

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

8 Citations (Scopus)

Abstract

Mapping the position and quantifying the level of 5-methylcytosine (m⁵C) as a modification in different types of cellular RNA is an important objective in the emerging field of epitranscriptomics. Bisulfite conversion has long been the gold standard for detection of m⁵C in DNA but it can also be applied to RNA. Here, we detail methods for bisulfite treatment of RNA, locus-specific PCR amplification and detection of candidate sites by sequencing on the Illumina MiSeq platform.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	269-284
Number of pages	16
DOIs	https://doi.org/10.1007/978-1-4939-3067-8_16
Publication status	Published - 2016

Publication series

Name	Methods in Molecular Biology
Volume	1358
ISSN (Print)	1064-3745

Access to Document

10.1007/978-1-4939-3067-8_16

Cite this

@inbook{79494eba7eac481287ae1aae0e068845,

title = "Nucleotide-level profiling of M5C RNA methylation",

abstract = "Mapping the position and quantifying the level of 5-methylcytosine (m5C) as a modification in different types of cellular RNA is an important objective in the emerging field of epitranscriptomics. Bisulfite conversion has long been the gold standard for detection of m5C in DNA but it can also be applied to RNA. Here, we detail methods for bisulfite treatment of RNA, locus-specific PCR amplification and detection of candidate sites by sequencing on the Illumina MiSeq platform.",

keywords = "5-Methylcytosine, Bisulfite conversion, Epitranscriptomics, MiSeq, Next-generation sequencing",

author = "Tennille Sibbritt and Andrew Shafik and Clark, {Susan J.} and Thomas Preiss",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016",

year = "2016",

doi = "10.1007/978-1-4939-3067-8_16",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "269--284",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Nucleotide-level profiling of M5C RNA methylation

AU - Sibbritt, Tennille

AU - Shafik, Andrew

AU - Clark, Susan J.

AU - Preiss, Thomas

N1 - Publisher Copyright: © Springer Science+Business Media New York 2016

PY - 2016

Y1 - 2016

N2 - Mapping the position and quantifying the level of 5-methylcytosine (m5C) as a modification in different types of cellular RNA is an important objective in the emerging field of epitranscriptomics. Bisulfite conversion has long been the gold standard for detection of m5C in DNA but it can also be applied to RNA. Here, we detail methods for bisulfite treatment of RNA, locus-specific PCR amplification and detection of candidate sites by sequencing on the Illumina MiSeq platform.

AB - Mapping the position and quantifying the level of 5-methylcytosine (m5C) as a modification in different types of cellular RNA is an important objective in the emerging field of epitranscriptomics. Bisulfite conversion has long been the gold standard for detection of m5C in DNA but it can also be applied to RNA. Here, we detail methods for bisulfite treatment of RNA, locus-specific PCR amplification and detection of candidate sites by sequencing on the Illumina MiSeq platform.

KW - 5-Methylcytosine

KW - Bisulfite conversion

KW - Epitranscriptomics

KW - MiSeq

KW - Next-generation sequencing

UR - http://www.scopus.com/inward/record.url?scp=84944474719&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3067-8_16

DO - 10.1007/978-1-4939-3067-8_16

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 269

EP - 284

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Nucleotide-level profiling of M⁵C RNA methylation

Abstract

Publication series

Access to Document

Other files and links

Fingerprint

Cite this