Optical properties of xanthene based fluorescent dyes studied by stretched-film linear dichroism

Kasra Razmkhah; Haydn Little; Sandeep Sandhu; Timothy R. Dafforn; Alison Rodger

doi:10.1039/c4ra06126h

Optical properties of xanthene based fluorescent dyes studied by stretched-film linear dichroism

Kasra Razmkhah, Haydn Little, Sandeep Sandhu, Timothy R. Dafforn, Alison Rodger^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Xanthene dyes are commonly used to label proteins in order to probe their location and activity using fluorescence spectroscopy and microscopy. However, fundamental properties such as the polarizations of transitions for many of the dyes have not been available. In this paper we report the use of recently developed oxidised polyethylene (PE^OX) stretched film linear dichroism to determine the transition polarizations of xanthene, 9-methyl-2,3,7-trihydroxy-6-fluorone, pyronin Y, pyronin B, fluorescein, and rhodamine 6G. The effect of the formation of higher order structures is also discussed when they occur. The dyes (except xanthene) all have an intense long-axis polarized transition in the region of 500 nm. They also have long-axis (//) polarized transitions from about 280 nm downwards in wavelength. There are suggestions of a weak short axis (bottom) polarized transition in the region of 320-350 nm in each case.

Original language	English
Pages (from-to)	37510-37515
Number of pages	6
Journal	RSC Advances
Volume	4
Issue number	71
DOIs	https://doi.org/10.1039/c4ra06126h
Publication status	Published - 2014
Externally published	Yes

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abstract = "Xanthene dyes are commonly used to label proteins in order to probe their location and activity using fluorescence spectroscopy and microscopy. However, fundamental properties such as the polarizations of transitions for many of the dyes have not been available. In this paper we report the use of recently developed oxidised polyethylene (PEOX) stretched film linear dichroism to determine the transition polarizations of xanthene, 9-methyl-2,3,7-trihydroxy-6-fluorone, pyronin Y, pyronin B, fluorescein, and rhodamine 6G. The effect of the formation of higher order structures is also discussed when they occur. The dyes (except xanthene) all have an intense long-axis polarized transition in the region of 500 nm. They also have long-axis (//) polarized transitions from about 280 nm downwards in wavelength. There are suggestions of a weak short axis (bottom) polarized transition in the region of 320-350 nm in each case.",

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AU - Razmkhah, Kasra

AU - Little, Haydn

AU - Sandhu, Sandeep

AU - Dafforn, Timothy R.

AU - Rodger, Alison

PY - 2014

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N2 - Xanthene dyes are commonly used to label proteins in order to probe their location and activity using fluorescence spectroscopy and microscopy. However, fundamental properties such as the polarizations of transitions for many of the dyes have not been available. In this paper we report the use of recently developed oxidised polyethylene (PEOX) stretched film linear dichroism to determine the transition polarizations of xanthene, 9-methyl-2,3,7-trihydroxy-6-fluorone, pyronin Y, pyronin B, fluorescein, and rhodamine 6G. The effect of the formation of higher order structures is also discussed when they occur. The dyes (except xanthene) all have an intense long-axis polarized transition in the region of 500 nm. They also have long-axis (//) polarized transitions from about 280 nm downwards in wavelength. There are suggestions of a weak short axis (bottom) polarized transition in the region of 320-350 nm in each case.

AB - Xanthene dyes are commonly used to label proteins in order to probe their location and activity using fluorescence spectroscopy and microscopy. However, fundamental properties such as the polarizations of transitions for many of the dyes have not been available. In this paper we report the use of recently developed oxidised polyethylene (PEOX) stretched film linear dichroism to determine the transition polarizations of xanthene, 9-methyl-2,3,7-trihydroxy-6-fluorone, pyronin Y, pyronin B, fluorescein, and rhodamine 6G. The effect of the formation of higher order structures is also discussed when they occur. The dyes (except xanthene) all have an intense long-axis polarized transition in the region of 500 nm. They also have long-axis (//) polarized transitions from about 280 nm downwards in wavelength. There are suggestions of a weak short axis (bottom) polarized transition in the region of 320-350 nm in each case.

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Optical properties of xanthene based fluorescent dyes studied by stretched-film linear dichroism

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