Optical trapping and surgery of living yeast cells using a single laser

Jun Ando, Godofredo Bautista, Nicholas Smith, Katsumasa Fujita, Vincent Ricardo Daria

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

We present optical trapping and surgery of living yeast cells using two operational modes of a single laser. We used a focused laser beam operating in continuous-wave mode for noninvasive optical trapping and manipulation of single yeast cell. We verified that such operational mode of the laser does not cause any destructive effect on yeast cell wall. By changing the operation of the laser to femtosecond-pulsed mode, we show that a tightly focused beam dissects the yeast cell walls via nonlinear absorption. Lastly, using the combined technique of optical microsurgery and trapping, we demonstrate intracellular organelle extraction and manipulation from a yeast cell. The technique established here will be useful as an efficient method for both surgery and manipulation of living cells using a single laser beam.

Original languageEnglish
Article number103705
JournalReview of Scientific Instruments
Volume79
Issue number10
DOIs
Publication statusPublished - 2008
Externally publishedYes

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