Optimal functional imaging of dendritic activity via oblique single-photon excitation of voltage indicators

Michael Castanares, Hans A. Bachor, Vincent Daria

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We use patterned light to initiate scattered photons that are obliquely directed to excite voltage indicators, which report changes in the membrane potential. Our proposed scheme reduces the excitation of voltage indicators elsewhere in the cell that constitutes the background signal and therefore enables us to amplify the detected fluorescence carrying the voltage information.

Original languageEnglish
Title of host publicationBiomedical Imaging and Sensing Conference 2020
EditorsToyohiko Yatagai, Yoshihisa Aizu, Osamu Matoba, Yasuhiro Awatsuji, Yuan Luo
PublisherSPIE
ISBN (Electronic)9781510638495
DOIs
Publication statusPublished - 2020
Event6th Biomedical Imaging and Sensing Conference - Yokohama, Japan
Duration: 20 Apr 202024 Apr 2020

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11521
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

Conference6th Biomedical Imaging and Sensing Conference
Country/TerritoryJapan
CityYokohama
Period20/04/2024/04/20

Cite this