Organophosphate-degrading metallohydrolases: Structure and function of potent catalysts for applications in bioremediation

Gerhard Schenk; Irsa Mateen; Tee Kheang Ng; Marcelo M. Pedroso; Nataša Mitić; Miguel Jafelicci; Rodrigo F.C. Marques; Lawrence R. Gahan; David L. Ollis

doi:10.1016/j.ccr.2016.03.006

Organophosphate-degrading metallohydrolases: Structure and function of potent catalysts for applications in bioremediation

Gerhard Schenk^*, Irsa Mateen, Tee Kheang Ng, Marcelo M. Pedroso, Nataša Mitić, Miguel Jafelicci, Rodrigo F.C. Marques, Lawrence R. Gahan, David L. Ollis

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

78 Citations (Scopus)

Abstract

Organophosphate compounds (OPs) have been employed in the agricultural industry as pesticides and insecticides for several decades. Many of the methods used currently for the detoxification of OPs are harmful and possess serious environmental consequences. Therefore, utilizing enzymes for the detection and decontamination of OPs is gaining increasing attention as an efficient and clean bioremediation strategy. Microbial enzymes, such as OP hydrolases, OP acid anhydrolases or methyl parathion hydrolase (MPH), are potent agents for OP decontamination. Their biochemical properties and biotechnological applications are discussed in this review, including a discussion on methods that may be employed to immobilize such enzymes, and essential steps to generate reusable and affordable biocatalytic systems for use in bioremediation and biorestoration.

Original language	English
Pages (from-to)	122-131
Number of pages	10
Journal	Coordination Chemistry Reviews
Volume	317
DOIs	https://doi.org/10.1016/j.ccr.2016.03.006
Publication status	Published - 15 Jun 2016

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title = "Organophosphate-degrading metallohydrolases: Structure and function of potent catalysts for applications in bioremediation",

abstract = "Organophosphate compounds (OPs) have been employed in the agricultural industry as pesticides and insecticides for several decades. Many of the methods used currently for the detoxification of OPs are harmful and possess serious environmental consequences. Therefore, utilizing enzymes for the detection and decontamination of OPs is gaining increasing attention as an efficient and clean bioremediation strategy. Microbial enzymes, such as OP hydrolases, OP acid anhydrolases or methyl parathion hydrolase (MPH), are potent agents for OP decontamination. Their biochemical properties and biotechnological applications are discussed in this review, including a discussion on methods that may be employed to immobilize such enzymes, and essential steps to generate reusable and affordable biocatalytic systems for use in bioremediation and biorestoration.",

keywords = "Bioremediation, Catalysis, Enzyme immobilization, Metallohydrolases, Organophosphate pesticides and nerve agents",

author = "Gerhard Schenk and Irsa Mateen and Ng, {Tee Kheang} and Pedroso, {Marcelo M.} and Nata{\v s}a Miti{\'c} and Miguel Jafelicci and Marques, {Rodrigo F.C.} and Gahan, {Lawrence R.} and Ollis, {David L.}",

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year = "2016",

month = jun,

day = "15",

doi = "10.1016/j.ccr.2016.03.006",

language = "English",

volume = "317",

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journal = "Coordination Chemistry Reviews",

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T1 - Organophosphate-degrading metallohydrolases

T2 - Structure and function of potent catalysts for applications in bioremediation

AU - Schenk, Gerhard

AU - Mateen, Irsa

AU - Ng, Tee Kheang

AU - Pedroso, Marcelo M.

AU - Mitić, Nataša

AU - Jafelicci, Miguel

AU - Marques, Rodrigo F.C.

AU - Gahan, Lawrence R.

AU - Ollis, David L.

PY - 2016/6/15

Y1 - 2016/6/15

N2 - Organophosphate compounds (OPs) have been employed in the agricultural industry as pesticides and insecticides for several decades. Many of the methods used currently for the detoxification of OPs are harmful and possess serious environmental consequences. Therefore, utilizing enzymes for the detection and decontamination of OPs is gaining increasing attention as an efficient and clean bioremediation strategy. Microbial enzymes, such as OP hydrolases, OP acid anhydrolases or methyl parathion hydrolase (MPH), are potent agents for OP decontamination. Their biochemical properties and biotechnological applications are discussed in this review, including a discussion on methods that may be employed to immobilize such enzymes, and essential steps to generate reusable and affordable biocatalytic systems for use in bioremediation and biorestoration.

AB - Organophosphate compounds (OPs) have been employed in the agricultural industry as pesticides and insecticides for several decades. Many of the methods used currently for the detoxification of OPs are harmful and possess serious environmental consequences. Therefore, utilizing enzymes for the detection and decontamination of OPs is gaining increasing attention as an efficient and clean bioremediation strategy. Microbial enzymes, such as OP hydrolases, OP acid anhydrolases or methyl parathion hydrolase (MPH), are potent agents for OP decontamination. Their biochemical properties and biotechnological applications are discussed in this review, including a discussion on methods that may be employed to immobilize such enzymes, and essential steps to generate reusable and affordable biocatalytic systems for use in bioremediation and biorestoration.

KW - Bioremediation

KW - Catalysis

KW - Enzyme immobilization

KW - Metallohydrolases

KW - Organophosphate pesticides and nerve agents

UR - http://www.scopus.com/inward/record.url?scp=84961857199&partnerID=8YFLogxK

U2 - 10.1016/j.ccr.2016.03.006

DO - 10.1016/j.ccr.2016.03.006

M3 - Review article

SN - 0010-8545

VL - 317

SP - 122

EP - 131

JO - Coordination Chemistry Reviews

JF - Coordination Chemistry Reviews

ER -

Organophosphate-degrading metallohydrolases: Structure and function of potent catalysts for applications in bioremediation

Abstract

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