Paramagnetic labelling of proteins and oligonucleotides for NMR

Xun Cheng Su; Gottfried Otting

doi:10.1007/s10858-009-9331-1

Paramagnetic labelling of proteins and oligonucleotides for NMR

Xun Cheng Su, Gottfried Otting^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

154 Citations (Scopus)

Abstract

Paramagnetic effects offer a rich source of long-range structural restraints. Here we review current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic molecules. The paramagnetic tags include nitroxide radicals and metal chelators. Particular emphasis is placed on tags suitable for site-specific and rigid attachment of lanthanide ions to macromolecules.

Original language	English
Pages (from-to)	101-112
Number of pages	12
Journal	Journal of Biomolecular NMR
Volume	46
Issue number	1
DOIs	https://doi.org/10.1007/s10858-009-9331-1
Publication status	Published - Jan 2010

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10.1007/s10858-009-9331-1

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abstract = "Paramagnetic effects offer a rich source of long-range structural restraints. Here we review current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic molecules. The paramagnetic tags include nitroxide radicals and metal chelators. Particular emphasis is placed on tags suitable for site-specific and rigid attachment of lanthanide ions to macromolecules.",

keywords = "Lanthanides, Nitroxide Radicals, Paramagnetic NMR, Paramagnetic protein and oligonucleotide tags, Site-specific labelling",

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AB - Paramagnetic effects offer a rich source of long-range structural restraints. Here we review current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic molecules. The paramagnetic tags include nitroxide radicals and metal chelators. Particular emphasis is placed on tags suitable for site-specific and rigid attachment of lanthanide ions to macromolecules.

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KW - Nitroxide Radicals

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KW - Paramagnetic protein and oligonucleotide tags

KW - Site-specific labelling

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Paramagnetic labelling of proteins and oligonucleotides for NMR

Abstract

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