Patterned illumination for analyzing neuronal function in 3D

Mary Ann Goa*, Christian Strickera, Steve Redmana, Hans Bachorb, Vincent Ricardo Daria

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    We use patterned 3D multi-spot illumination to perform neuronal multi-site stimulation in rat brain tissue. Using a spatial light modulator, we holograpically project 3D light fields for multi-site two-photon photolysis of caged neurotransmitters to generate synaptic inputs to a neuron. Controlled photostimulation of multiple synapses from various locations in the dendritic tree provides a way to analyze how neurons integrate multiple inputs. Our holographic projection setup is incorporated into a two-photon 3D imaging microscope for visualization and for accurate positioning of specific uncaging sites along the neuron's dendritic tree. We show two-photon images and the neuron's response to holographic photostimulation of synapses along dendrites.

    Original languageEnglish
    Title of host publicationBiophotonics
    Subtitle of host publicationPhotonic Solutions for Better Health Care III
    DOIs
    Publication statusPublished - 2012
    EventBiophotonics: Photonic Solutions for Better Health Care III - Brussels, Belgium
    Duration: 16 Apr 201219 Apr 2012

    Publication series

    NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
    Volume8427
    ISSN (Print)1605-7422

    Conference

    ConferenceBiophotonics: Photonic Solutions for Better Health Care III
    Country/TerritoryBelgium
    CityBrussels
    Period16/04/1219/04/12

    Fingerprint

    Dive into the research topics of 'Patterned illumination for analyzing neuronal function in 3D'. Together they form a unique fingerprint.

    Cite this