TY - GEN
T1 - Patterned illumination for analyzing neuronal function in 3D
AU - Goa, Mary Ann
AU - Strickera, Christian
AU - Redmana, Steve
AU - Bachorb, Hans
AU - Daria, Vincent Ricardo
PY - 2012
Y1 - 2012
N2 - We use patterned 3D multi-spot illumination to perform neuronal multi-site stimulation in rat brain tissue. Using a spatial light modulator, we holograpically project 3D light fields for multi-site two-photon photolysis of caged neurotransmitters to generate synaptic inputs to a neuron. Controlled photostimulation of multiple synapses from various locations in the dendritic tree provides a way to analyze how neurons integrate multiple inputs. Our holographic projection setup is incorporated into a two-photon 3D imaging microscope for visualization and for accurate positioning of specific uncaging sites along the neuron's dendritic tree. We show two-photon images and the neuron's response to holographic photostimulation of synapses along dendrites.
AB - We use patterned 3D multi-spot illumination to perform neuronal multi-site stimulation in rat brain tissue. Using a spatial light modulator, we holograpically project 3D light fields for multi-site two-photon photolysis of caged neurotransmitters to generate synaptic inputs to a neuron. Controlled photostimulation of multiple synapses from various locations in the dendritic tree provides a way to analyze how neurons integrate multiple inputs. Our holographic projection setup is incorporated into a two-photon 3D imaging microscope for visualization and for accurate positioning of specific uncaging sites along the neuron's dendritic tree. We show two-photon images and the neuron's response to holographic photostimulation of synapses along dendrites.
KW - Holographic projection
KW - Synaptic integration
KW - Two-photon photolysis
UR - http://www.scopus.com/inward/record.url?scp=84861954967&partnerID=8YFLogxK
U2 - 10.1117/12.923421
DO - 10.1117/12.923421
M3 - Conference contribution
SN - 9780819491190
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Biophotonics
T2 - Biophotonics: Photonic Solutions for Better Health Care III
Y2 - 16 April 2012 through 19 April 2012
ER -