@inproceedings{a6d6f99311514e17998372e62b8cc59a,
title = "Patterned illumination for analyzing neuronal function in 3D",
abstract = "We use patterned 3D multi-spot illumination to perform neuronal multi-site stimulation in rat brain tissue. Using a spatial light modulator, we holograpically project 3D light fields for multi-site two-photon photolysis of caged neurotransmitters to generate synaptic inputs to a neuron. Controlled photostimulation of multiple synapses from various locations in the dendritic tree provides a way to analyze how neurons integrate multiple inputs. Our holographic projection setup is incorporated into a two-photon 3D imaging microscope for visualization and for accurate positioning of specific uncaging sites along the neuron's dendritic tree. We show two-photon images and the neuron's response to holographic photostimulation of synapses along dendrites.",
keywords = "Holographic projection, Synaptic integration, Two-photon photolysis",
author = "Goa, \{Mary Ann\} and Christian Strickera and Steve Redmana and Hans Bachorb and Daria, \{Vincent Ricardo\}",
year = "2012",
doi = "10.1117/12.923421",
language = "English",
isbn = "9780819491190",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Biophotonics",
note = "Biophotonics: Photonic Solutions for Better Health Care III ; Conference date: 16-04-2012 Through 19-04-2012",
}