PEA-CLARITY: 3D molecular imaging of whole plant organs

William M. Palmer*, Antony P. Martin, Jamie R. Flynn, Stephanie L. Reed, Rosemary G. White, Robert T. Furbank, Christopher P.L. Grof

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

Here we report the adaptation of the CLARITY technique to plant tissues with addition of enzymatic degradation to improve optical clearing and facilitate antibody probe penetration. Plant-Enzyme-Assisted (PEA)-CLARITY, has allowed deep optical visualisation of stains, expressed fluorescent proteins and IgG-antibodies in Tobacco and Arabidopsis leaves. Enzyme treatment enabled penetration of antibodies into whole tissues without the need for any sectioning of the material, thus facilitating protein localisation of intact tissue in 3D whilst retaining cellular structure.

Original languageEnglish
Article number13492
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 2 Sept 2015
Externally publishedYes

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