Peripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay

Hilary S. Warren; Fan Wu; Peggy L. Horn; David B. Pyne; Nicholas P. West; Allan W. Cripps

doi:10.1016/j.jim.2013.03.010

Peripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay

Hilary S. Warren^*, Fan Wu, Peggy L. Horn, David B. Pyne, Nicholas P. West, Allan W. Cripps

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

In this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NK cell function. Loss of NK cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11% for natural killing and 3% for antibody-dependent killing, compared to 14% and 9% respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NK cells in the population both for natural killing (CV 33%) and antibody-dependent killing (CV 27%).

Original language	English
Pages (from-to)	68-70
Number of pages	3
Journal	Journal of Immunological Methods
Volume	392
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2013.03.010
Publication status	Published - 28 Jun 2013

Access to Document

10.1016/j.jim.2013.03.010

Cite this

@article{eebfb44b6cc945cc98d3d6bf836f9284,

title = "Peripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay",

abstract = "In this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NK cell function. Loss of NK cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11% for natural killing and 3% for antibody-dependent killing, compared to 14% and 9% respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NK cells in the population both for natural killing (CV 33%) and antibody-dependent killing (CV 27%).",

keywords = "ADCC, Cytotoxicity, Human NK cells, Rituximab, TINKL",

author = "Warren, {Hilary S.} and Fan Wu and Horn, {Peggy L.} and Pyne, {David B.} and West, {Nicholas P.} and Cripps, {Allan W.}",

year = "2013",

month = jun,

day = "28",

doi = "10.1016/j.jim.2013.03.010",

language = "English",

volume = "392",

pages = "68--70",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Peripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay

AU - Warren, Hilary S.

AU - Wu, Fan

AU - Horn, Peggy L.

AU - Pyne, David B.

AU - West, Nicholas P.

AU - Cripps, Allan W.

PY - 2013/6/28

Y1 - 2013/6/28

N2 - In this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NK cell function. Loss of NK cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11% for natural killing and 3% for antibody-dependent killing, compared to 14% and 9% respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NK cells in the population both for natural killing (CV 33%) and antibody-dependent killing (CV 27%).

AB - In this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NK cell function. Loss of NK cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11% for natural killing and 3% for antibody-dependent killing, compared to 14% and 9% respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NK cells in the population both for natural killing (CV 33%) and antibody-dependent killing (CV 27%).

KW - ADCC

KW - Cytotoxicity

KW - Human NK cells

KW - Rituximab

KW - TINKL

UR - http://www.scopus.com/inward/record.url?scp=84880042895&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2013.03.010

DO - 10.1016/j.jim.2013.03.010

M3 - Article

SN - 0022-1759

VL - 392

SP - 68

EP - 70

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Peripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay

Abstract

Access to Document

Other files and links

Fingerprint

Cite this