TY - JOUR
T1 - Plasmodium falciparum culture
T2 - The benefits of shaking
AU - Allen, Richard J.W.
AU - Kirk, Kiaran
PY - 2010/1
Y1 - 2010/1
N2 - Despite evidence that the suspension of malaria cultures leads to improved parasite growth, the practice of culturing the parasite under static conditions remains widespread. Here, extending previous work, we have quantified the favourable effects of continuous agitation on three indices of culture growth: (i) parasite yield, (ii) culture synchrony after a synchronisation procedure, and (iii) the prevalence of multiple infections. In addition, we show that under continuous suspension, the time taken for genetically altered parasites to re-populate cultures post-transfection is dramatically reduced.
AB - Despite evidence that the suspension of malaria cultures leads to improved parasite growth, the practice of culturing the parasite under static conditions remains widespread. Here, extending previous work, we have quantified the favourable effects of continuous agitation on three indices of culture growth: (i) parasite yield, (ii) culture synchrony after a synchronisation procedure, and (iii) the prevalence of multiple infections. In addition, we show that under continuous suspension, the time taken for genetically altered parasites to re-populate cultures post-transfection is dramatically reduced.
KW - Culture
KW - Multiply infected erythrocytes
KW - Shaking
KW - Static
KW - Synchronisation
KW - Transfected parasites
UR - http://www.scopus.com/inward/record.url?scp=71549139434&partnerID=8YFLogxK
U2 - 10.1016/j.molbiopara.2009.09.005
DO - 10.1016/j.molbiopara.2009.09.005
M3 - Article
SN - 0166-6851
VL - 169
SP - 63
EP - 65
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1
ER -