TY - JOUR
T1 - Plasmodium-infected erythrocytes induce secretion of IGFBP7 to form type ii rosettes and escape phagocytosis
AU - Lee, Wenn Chyau
AU - Russell, Bruce
AU - Sobota, Radoslaw Mikolaj
AU - Ghaffar, Khairunnisa
AU - Howland, Shanshan W.
AU - Wong, Zi Xin
AU - Maier, Alexander G.
AU - Dorin-Semblat, Dominique
AU - Biswas, Subhra
AU - Gamain, Benoit
AU - Lau, Yee Ling
AU - Malleret, Benoit
AU - Chu, Cindy
AU - Nosten, François
AU - Renia, Laurent
N1 - Publisher Copyright:
© Lee et al.
PY - 2020/2
Y1 - 2020/2
N2 - In malaria, rosetting is described as a phenomenon where an infected erythrocyte (IRBC) is attached to uninfected erythrocytes (URBC). In some studies, rosetting has been associated with malaria pathogenesis. Here, we have identified a new type of rosetting. Using a step-by-step approach, we identified IGFBP7, a protein secreted by monocytes in response to parasite stimulation, as a rosette-stimulator for Plasmodium falciparum- and P. vivax-IRBC. IGFBP7-mediated rosette-stimulation was rapid yet reversible. Unlike type I rosetting that involves direct interaction of rosetting ligands on IRBC and receptors on URBC, the IGFBP7-mediated, type II rosetting requires two additional serum factors, namely von Willebrand factor and thrombospondin-1. These two factors interact with IGFBP7 to mediate rosette formation by the IRBC. Importantly, the IGFBP7-induced type II rosetting hampers phagocytosis of IRBC by host phagocytes.
AB - In malaria, rosetting is described as a phenomenon where an infected erythrocyte (IRBC) is attached to uninfected erythrocytes (URBC). In some studies, rosetting has been associated with malaria pathogenesis. Here, we have identified a new type of rosetting. Using a step-by-step approach, we identified IGFBP7, a protein secreted by monocytes in response to parasite stimulation, as a rosette-stimulator for Plasmodium falciparum- and P. vivax-IRBC. IGFBP7-mediated rosette-stimulation was rapid yet reversible. Unlike type I rosetting that involves direct interaction of rosetting ligands on IRBC and receptors on URBC, the IGFBP7-mediated, type II rosetting requires two additional serum factors, namely von Willebrand factor and thrombospondin-1. These two factors interact with IGFBP7 to mediate rosette formation by the IRBC. Importantly, the IGFBP7-induced type II rosetting hampers phagocytosis of IRBC by host phagocytes.
UR - http://www.scopus.com/inward/record.url?scp=85081069101&partnerID=8YFLogxK
U2 - 10.7554/eLife.51546
DO - 10.7554/eLife.51546
M3 - Article
SN - 2050-084X
VL - 9
JO - eLife
JF - eLife
M1 - e51546
ER -