Plasmodium-infected erythrocytes induce secretion of IGFBP7 to form type ii rosettes and escape phagocytosis

Wenn Chyau Lee; Bruce Russell; Radoslaw Mikolaj Sobota; Khairunnisa Ghaffar; Shanshan W. Howland; Zi Xin Wong; Alexander G. Maier; Dominique Dorin-Semblat; Subhra Biswas; Benoit Gamain; Yee Ling Lau; Benoit Malleret; Cindy Chu; François Nosten; Laurent Renia

doi:10.7554/eLife.51546

Plasmodium-infected erythrocytes induce secretion of IGFBP7 to form type ii rosettes and escape phagocytosis

Wenn Chyau Lee, Bruce Russell, Radoslaw Mikolaj Sobota, Khairunnisa Ghaffar, Shanshan W. Howland, Zi Xin Wong, Alexander G. Maier, Dominique Dorin-Semblat, Subhra Biswas, Benoit Gamain, Yee Ling Lau, Benoit Malleret, Cindy Chu, François Nosten, Laurent Renia^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

In malaria, rosetting is described as a phenomenon where an infected erythrocyte (IRBC) is attached to uninfected erythrocytes (URBC). In some studies, rosetting has been associated with malaria pathogenesis. Here, we have identified a new type of rosetting. Using a step-by-step approach, we identified IGFBP7, a protein secreted by monocytes in response to parasite stimulation, as a rosette-stimulator for Plasmodium falciparum- and P. vivax-IRBC. IGFBP7-mediated rosette-stimulation was rapid yet reversible. Unlike type I rosetting that involves direct interaction of rosetting ligands on IRBC and receptors on URBC, the IGFBP7-mediated, type II rosetting requires two additional serum factors, namely von Willebrand factor and thrombospondin-1. These two factors interact with IGFBP7 to mediate rosette formation by the IRBC. Importantly, the IGFBP7-induced type II rosetting hampers phagocytosis of IRBC by host phagocytes.

Original language	English
Article number	e51546
Journal	eLife
Volume	9
DOIs	https://doi.org/10.7554/eLife.51546
Publication status	Published - Feb 2020

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10.7554/eLife.51546

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Lee, W. C., Russell, B., Sobota, R. M., Ghaffar, K., Howland, S. W., Wong, Z. X., Maier, A. G., Dorin-Semblat, D., Biswas, S., Gamain, B., Lau, Y. L., Malleret, B., Chu, C., Nosten, F., & Renia, L. (2020). Plasmodium-infected erythrocytes induce secretion of IGFBP7 to form type ii rosettes and escape phagocytosis. eLife, 9, Article e51546. https://doi.org/10.7554/eLife.51546

@article{e5fb661971b740e2a93ca54e210bd9c8,

title = "Plasmodium-infected erythrocytes induce secretion of IGFBP7 to form type ii rosettes and escape phagocytosis",

abstract = "In malaria, rosetting is described as a phenomenon where an infected erythrocyte (IRBC) is attached to uninfected erythrocytes (URBC). In some studies, rosetting has been associated with malaria pathogenesis. Here, we have identified a new type of rosetting. Using a step-by-step approach, we identified IGFBP7, a protein secreted by monocytes in response to parasite stimulation, as a rosette-stimulator for Plasmodium falciparum- and P. vivax-IRBC. IGFBP7-mediated rosette-stimulation was rapid yet reversible. Unlike type I rosetting that involves direct interaction of rosetting ligands on IRBC and receptors on URBC, the IGFBP7-mediated, type II rosetting requires two additional serum factors, namely von Willebrand factor and thrombospondin-1. These two factors interact with IGFBP7 to mediate rosette formation by the IRBC. Importantly, the IGFBP7-induced type II rosetting hampers phagocytosis of IRBC by host phagocytes.",

author = "Lee, \{Wenn Chyau\} and Bruce Russell and Sobota, \{Radoslaw Mikolaj\} and Khairunnisa Ghaffar and Howland, \{Shanshan W.\} and Wong, \{Zi Xin\} and Maier, \{Alexander G.\} and Dominique Dorin-Semblat and Subhra Biswas and Benoit Gamain and Lau, \{Yee Ling\} and Benoit Malleret and Cindy Chu and Fran{\c c}ois Nosten and Laurent Renia",

note = "Publisher Copyright: {\textcopyright} Lee et al.",

year = "2020",

month = feb,

doi = "10.7554/eLife.51546",

language = "English",

volume = "9",

journal = "eLife",

issn = "2050-084X",

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T1 - Plasmodium-infected erythrocytes induce secretion of IGFBP7 to form type ii rosettes and escape phagocytosis

AU - Lee, Wenn Chyau

AU - Russell, Bruce

AU - Sobota, Radoslaw Mikolaj

AU - Ghaffar, Khairunnisa

AU - Howland, Shanshan W.

AU - Wong, Zi Xin

AU - Maier, Alexander G.

AU - Dorin-Semblat, Dominique

AU - Biswas, Subhra

AU - Gamain, Benoit

AU - Lau, Yee Ling

AU - Malleret, Benoit

AU - Chu, Cindy

AU - Nosten, François

AU - Renia, Laurent

PY - 2020/2

Y1 - 2020/2

N2 - In malaria, rosetting is described as a phenomenon where an infected erythrocyte (IRBC) is attached to uninfected erythrocytes (URBC). In some studies, rosetting has been associated with malaria pathogenesis. Here, we have identified a new type of rosetting. Using a step-by-step approach, we identified IGFBP7, a protein secreted by monocytes in response to parasite stimulation, as a rosette-stimulator for Plasmodium falciparum- and P. vivax-IRBC. IGFBP7-mediated rosette-stimulation was rapid yet reversible. Unlike type I rosetting that involves direct interaction of rosetting ligands on IRBC and receptors on URBC, the IGFBP7-mediated, type II rosetting requires two additional serum factors, namely von Willebrand factor and thrombospondin-1. These two factors interact with IGFBP7 to mediate rosette formation by the IRBC. Importantly, the IGFBP7-induced type II rosetting hampers phagocytosis of IRBC by host phagocytes.

AB - In malaria, rosetting is described as a phenomenon where an infected erythrocyte (IRBC) is attached to uninfected erythrocytes (URBC). In some studies, rosetting has been associated with malaria pathogenesis. Here, we have identified a new type of rosetting. Using a step-by-step approach, we identified IGFBP7, a protein secreted by monocytes in response to parasite stimulation, as a rosette-stimulator for Plasmodium falciparum- and P. vivax-IRBC. IGFBP7-mediated rosette-stimulation was rapid yet reversible. Unlike type I rosetting that involves direct interaction of rosetting ligands on IRBC and receptors on URBC, the IGFBP7-mediated, type II rosetting requires two additional serum factors, namely von Willebrand factor and thrombospondin-1. These two factors interact with IGFBP7 to mediate rosette formation by the IRBC. Importantly, the IGFBP7-induced type II rosetting hampers phagocytosis of IRBC by host phagocytes.

UR - https://www.scopus.com/pages/publications/85081069101

U2 - 10.7554/eLife.51546

DO - 10.7554/eLife.51546

M3 - Article

SN - 2050-084X

VL - 9

JO - eLife

JF - eLife

M1 - e51546

ER -

Plasmodium-infected erythrocytes induce secretion of IGFBP7 to form type ii rosettes and escape phagocytosis

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