TY - JOUR
T1 - Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children
AU - Strachan, Roxanne E.
AU - Cornelius, Anita
AU - Gilbert, Gwendolyn L.
AU - Gulliver, Tanya
AU - Martin, Andrew
AU - McDonald, Tim
AU - Nixon, Gillian
AU - Roseby, Rob
AU - Ranganathan, Sarath
AU - Selvadurai, Hiran
AU - Smith, Greg
AU - Soto-Martinez, Manuel
AU - Suresh, Sadasivam
AU - Teoh, Laurel
AU - Thapa, Kiran
AU - Wainwright, Claire E.
AU - Jaffé, Adam
PY - 2012/1
Y1 - 2012/1
N2 - Background and objective: National surveillance of invasive pneumococcal disease (IPD) includes serotyping Streptococcus pneumoniae (SP) isolates from sterile site cultures. PCR is more sensitive and can identify more SP serotypes (STs) in culture-negative samples. The aim of this study was to determine whether enhanced surveillance of childhood empyema, using PCR, provides additional serotype information compared with conventional surveillance. Methods: Pleural fluid (PF) from children with empyema were cultured and tested by PCR to identify SP, targeting the autolysin gene (lytA). Multiplex PCR-based reverse line blot assay was used to identify SP STs. Corresponding IPD surveillance and serotype data were obtained from the National Notifiable Diseases Surveillance System (NNDSS). Results: Eighty-nine children with empyema, aged ≤16 years, were recruited between April 2008 and March 2009, inclusive. SP was isolated from 5/84 (5.9%) PF cultures and by PCR in 43/79 (54.4%) PF samples.
AB - Background and objective: National surveillance of invasive pneumococcal disease (IPD) includes serotyping Streptococcus pneumoniae (SP) isolates from sterile site cultures. PCR is more sensitive and can identify more SP serotypes (STs) in culture-negative samples. The aim of this study was to determine whether enhanced surveillance of childhood empyema, using PCR, provides additional serotype information compared with conventional surveillance. Methods: Pleural fluid (PF) from children with empyema were cultured and tested by PCR to identify SP, targeting the autolysin gene (lytA). Multiplex PCR-based reverse line blot assay was used to identify SP STs. Corresponding IPD surveillance and serotype data were obtained from the National Notifiable Diseases Surveillance System (NNDSS). Results: Eighty-nine children with empyema, aged ≤16 years, were recruited between April 2008 and March 2009, inclusive. SP was isolated from 5/84 (5.9%) PF cultures and by PCR in 43/79 (54.4%) PF samples.
KW - empyema
KW - paediatrics
KW - pleural disease
KW - pneumococcal surveillance
KW - respiratory infections
UR - http://www.scopus.com/inward/record.url?scp=84255177052&partnerID=8YFLogxK
U2 - 10.1111/j.1440-1843.2011.02035.x
DO - 10.1111/j.1440-1843.2011.02035.x
M3 - Article
SN - 1323-7799
VL - 17
SP - 114
EP - 119
JO - Respirology
JF - Respirology
IS - 1
ER -