Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children

Roxanne E. Strachan; Anita Cornelius; Gwendolyn L. Gilbert; Tanya Gulliver; Andrew Martin; Tim McDonald; Gillian Nixon; Rob Roseby; Sarath Ranganathan; Hiran Selvadurai; Greg Smith; Manuel Soto-Martinez; Sadasivam Suresh; Laurel Teoh; Kiran Thapa; Claire E. Wainwright; Adam Jaffé

doi:10.1111/j.1440-1843.2011.02035.x

Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children

Roxanne E. Strachan, Anita Cornelius, Gwendolyn L. Gilbert, Tanya Gulliver, Andrew Martin, Tim McDonald, Gillian Nixon, Rob Roseby, Sarath Ranganathan, Hiran Selvadurai, Greg Smith, Manuel Soto-Martinez, Sadasivam Suresh, Laurel Teoh, Kiran Thapa, Claire E. Wainwright, Adam Jaffé^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Background and objective: National surveillance of invasive pneumococcal disease (IPD) includes serotyping Streptococcus pneumoniae (SP) isolates from sterile site cultures. PCR is more sensitive and can identify more SP serotypes (STs) in culture-negative samples. The aim of this study was to determine whether enhanced surveillance of childhood empyema, using PCR, provides additional serotype information compared with conventional surveillance. Methods: Pleural fluid (PF) from children with empyema were cultured and tested by PCR to identify SP, targeting the autolysin gene (lytA). Multiplex PCR-based reverse line blot assay was used to identify SP STs. Corresponding IPD surveillance and serotype data were obtained from the National Notifiable Diseases Surveillance System (NNDSS). Results: Eighty-nine children with empyema, aged ≤16 years, were recruited between April 2008 and March 2009, inclusive. SP was isolated from 5/84 (5.9%) PF cultures and by PCR in 43/79 (54.4%) PF samples.

Original language	English
Pages (from-to)	114-119
Number of pages	6
Journal	Respirology
Volume	17
Issue number	1
DOIs	https://doi.org/10.1111/j.1440-1843.2011.02035.x
Publication status	Published - Jan 2012
Externally published	Yes

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10.1111/j.1440-1843.2011.02035.x

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Strachan, R. E., Cornelius, A., Gilbert, G. L., Gulliver, T., Martin, A., McDonald, T., Nixon, G., Roseby, R., Ranganathan, S., Selvadurai, H., Smith, G., Soto-Martinez, M., Suresh, S., Teoh, L., Thapa, K., Wainwright, C. E., & Jaffé, A. (2012). Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children. Respirology, 17(1), 114-119. https://doi.org/10.1111/j.1440-1843.2011.02035.x

@article{a62c735e49a3496db87031494a6d0adb,

title = "Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children",

abstract = "Background and objective: National surveillance of invasive pneumococcal disease (IPD) includes serotyping Streptococcus pneumoniae (SP) isolates from sterile site cultures. PCR is more sensitive and can identify more SP serotypes (STs) in culture-negative samples. The aim of this study was to determine whether enhanced surveillance of childhood empyema, using PCR, provides additional serotype information compared with conventional surveillance. Methods: Pleural fluid (PF) from children with empyema were cultured and tested by PCR to identify SP, targeting the autolysin gene (lytA). Multiplex PCR-based reverse line blot assay was used to identify SP STs. Corresponding IPD surveillance and serotype data were obtained from the National Notifiable Diseases Surveillance System (NNDSS). Results: Eighty-nine children with empyema, aged ≤16 years, were recruited between April 2008 and March 2009, inclusive. SP was isolated from 5/84 (5.9\%) PF cultures and by PCR in 43/79 (54.4\%) PF samples.",

keywords = "empyema, paediatrics, pleural disease, pneumococcal surveillance, respiratory infections",

author = "Strachan, \{Roxanne E.\} and Anita Cornelius and Gilbert, \{Gwendolyn L.\} and Tanya Gulliver and Andrew Martin and Tim McDonald and Gillian Nixon and Rob Roseby and Sarath Ranganathan and Hiran Selvadurai and Greg Smith and Manuel Soto-Martinez and Sadasivam Suresh and Laurel Teoh and Kiran Thapa and Wainwright, \{Claire E.\} and Adam Jaff{\'e}",

year = "2012",

month = jan,

doi = "10.1111/j.1440-1843.2011.02035.x",

language = "English",

volume = "17",

pages = "114--119",

journal = "Respirology",

issn = "1323-7799",

publisher = "John Wiley \& Sons Inc.",

number = "1",

}

Strachan, RE, Cornelius, A, Gilbert, GL, Gulliver, T, Martin, A, McDonald, T, Nixon, G, Roseby, R, Ranganathan, S, Selvadurai, H, Smith, G, Soto-Martinez, M, Suresh, S, Teoh, L, Thapa, K, Wainwright, CE & Jaffé, A 2012, 'Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children', Respirology, vol. 17, no. 1, pp. 114-119. https://doi.org/10.1111/j.1440-1843.2011.02035.x

TY - JOUR

T1 - Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children

AU - Strachan, Roxanne E.

AU - Cornelius, Anita

AU - Gilbert, Gwendolyn L.

AU - Gulliver, Tanya

AU - Martin, Andrew

AU - McDonald, Tim

AU - Nixon, Gillian

AU - Roseby, Rob

AU - Ranganathan, Sarath

AU - Selvadurai, Hiran

AU - Smith, Greg

AU - Soto-Martinez, Manuel

AU - Suresh, Sadasivam

AU - Teoh, Laurel

AU - Thapa, Kiran

AU - Wainwright, Claire E.

AU - Jaffé, Adam

PY - 2012/1

Y1 - 2012/1

N2 - Background and objective: National surveillance of invasive pneumococcal disease (IPD) includes serotyping Streptococcus pneumoniae (SP) isolates from sterile site cultures. PCR is more sensitive and can identify more SP serotypes (STs) in culture-negative samples. The aim of this study was to determine whether enhanced surveillance of childhood empyema, using PCR, provides additional serotype information compared with conventional surveillance. Methods: Pleural fluid (PF) from children with empyema were cultured and tested by PCR to identify SP, targeting the autolysin gene (lytA). Multiplex PCR-based reverse line blot assay was used to identify SP STs. Corresponding IPD surveillance and serotype data were obtained from the National Notifiable Diseases Surveillance System (NNDSS). Results: Eighty-nine children with empyema, aged ≤16 years, were recruited between April 2008 and March 2009, inclusive. SP was isolated from 5/84 (5.9%) PF cultures and by PCR in 43/79 (54.4%) PF samples.

AB - Background and objective: National surveillance of invasive pneumococcal disease (IPD) includes serotyping Streptococcus pneumoniae (SP) isolates from sterile site cultures. PCR is more sensitive and can identify more SP serotypes (STs) in culture-negative samples. The aim of this study was to determine whether enhanced surveillance of childhood empyema, using PCR, provides additional serotype information compared with conventional surveillance. Methods: Pleural fluid (PF) from children with empyema were cultured and tested by PCR to identify SP, targeting the autolysin gene (lytA). Multiplex PCR-based reverse line blot assay was used to identify SP STs. Corresponding IPD surveillance and serotype data were obtained from the National Notifiable Diseases Surveillance System (NNDSS). Results: Eighty-nine children with empyema, aged ≤16 years, were recruited between April 2008 and March 2009, inclusive. SP was isolated from 5/84 (5.9%) PF cultures and by PCR in 43/79 (54.4%) PF samples.

KW - empyema

KW - paediatrics

KW - pleural disease

KW - pneumococcal surveillance

KW - respiratory infections

UR - https://www.scopus.com/pages/publications/84255177052

U2 - 10.1111/j.1440-1843.2011.02035.x

DO - 10.1111/j.1440-1843.2011.02035.x

M3 - Article

SN - 1323-7799

VL - 17

SP - 114

EP - 119

JO - Respirology

JF - Respirology

IS - 1

ER -

Pleural fluid nucleic acid testing enhances pneumococcal surveillance in children

Abstract

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