Polyadenylation State Microarray (PASTA) Analysis

Traude H. Beilharz, Thomas Preiss*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    4 Citations (Scopus)


    Nearly all eukaryotic mRNAs terminate in a poly(A) tail that serves important roles in mRNA utilization. In the cytoplasm, the poly(A) tail promotes both mRNA stability and translation, and these functions are frequently regulated through changes in tail length. To identify the scope of poly(A) tail length control in a transcriptome, we developed the polyadenylation state microarray (PASTA) method. It involves the purification of mRNA based on poly(A) tail length using thermal elution from poly(U) sepharose, followed by microarray analysis of the resulting fractions. In this chapter we detail our PASTA approach and describe some methods for bulk and mRNA-specific poly(A) tail length measurements of use to monitor the procedure and independently verify the microarray data.

    Original languageEnglish
    Title of host publicationYeast Systems Biology
    Subtitle of host publicationMethods and Protocols
    PublisherHumana Press Inc.
    Number of pages16
    ISBN (Print)9781617791727
    Publication statusPublished - 2011

    Publication series

    NameMethods in Molecular Biology
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029


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