Polymorphic Microsatellite loci for paternity analysis in the fiddler crab Uca mjoebergi

Mark Kinnear; Leon M.A. Smith; Golo Maurer; Pat R.Y. Backwell; Celeste C. Linde

doi:10.1651/08-3049R.1

Polymorphic Microsatellite loci for paternity analysis in the fiddler crab Uca mjoebergi

Mark Kinnear^*, Leon M.A. Smith, Golo Maurer, Pat R.Y. Backwell, Celeste C. Linde

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Microsatellite markers were developed for the fiddler crab Uca mjoebergi for use in paternity assignment. Five highly variable loci were found to amplify reliably in U. mjoebergi, although one locus exhibited evidence for the presence of null alleles, and two loci exhibited evidence of linkage. The number of alleles, expected heterozygosity and probability of paternity exclusion (P > 0.999) suggest that these markers when combined provide sufficient power to determine paternity by exclusion in this species. Three primer pairs were found to also amplify PCR products from three additional fiddler crab species (U. dampieri, U. flammula and U. seismella), and will prove useful for phylogeography and behavioural ecology of fiddler crabs.

Original language	English
Pages (from-to)	273-274
Number of pages	2
Journal	Journal of Crustacean Biology
Volume	29
Issue number	2
DOIs	https://doi.org/10.1651/08-3049R.1
Publication status	Published - May 2009

Access to Document

10.1651/08-3049R.1

Cite this

@article{57a236c16fd2438eb369d1c5a9aa6756,

title = "Polymorphic Microsatellite loci for paternity analysis in the fiddler crab Uca mjoebergi",

abstract = "Microsatellite markers were developed for the fiddler crab Uca mjoebergi for use in paternity assignment. Five highly variable loci were found to amplify reliably in U. mjoebergi, although one locus exhibited evidence for the presence of null alleles, and two loci exhibited evidence of linkage. The number of alleles, expected heterozygosity and probability of paternity exclusion (P > 0.999) suggest that these markers when combined provide sufficient power to determine paternity by exclusion in this species. Three primer pairs were found to also amplify PCR products from three additional fiddler crab species (U. dampieri, U. flammula and U. seismella), and will prove useful for phylogeography and behavioural ecology of fiddler crabs.",

keywords = "Fiddler crab, Microsatellite, Paternity, Polymorphism, Uca",

author = "Mark Kinnear and Smith, {Leon M.A.} and Golo Maurer and Backwell, {Pat R.Y.} and Linde, {Celeste C.}",

year = "2009",

month = may,

doi = "10.1651/08-3049R.1",

language = "English",

volume = "29",

pages = "273--274",

journal = "Journal of Crustacean Biology",

issn = "0278-0372",

publisher = "Brill Academic Publishers",

number = "2",

}

TY - JOUR

T1 - Polymorphic Microsatellite loci for paternity analysis in the fiddler crab Uca mjoebergi

AU - Kinnear, Mark

AU - Smith, Leon M.A.

AU - Maurer, Golo

AU - Backwell, Pat R.Y.

AU - Linde, Celeste C.

PY - 2009/5

Y1 - 2009/5

N2 - Microsatellite markers were developed for the fiddler crab Uca mjoebergi for use in paternity assignment. Five highly variable loci were found to amplify reliably in U. mjoebergi, although one locus exhibited evidence for the presence of null alleles, and two loci exhibited evidence of linkage. The number of alleles, expected heterozygosity and probability of paternity exclusion (P > 0.999) suggest that these markers when combined provide sufficient power to determine paternity by exclusion in this species. Three primer pairs were found to also amplify PCR products from three additional fiddler crab species (U. dampieri, U. flammula and U. seismella), and will prove useful for phylogeography and behavioural ecology of fiddler crabs.

AB - Microsatellite markers were developed for the fiddler crab Uca mjoebergi for use in paternity assignment. Five highly variable loci were found to amplify reliably in U. mjoebergi, although one locus exhibited evidence for the presence of null alleles, and two loci exhibited evidence of linkage. The number of alleles, expected heterozygosity and probability of paternity exclusion (P > 0.999) suggest that these markers when combined provide sufficient power to determine paternity by exclusion in this species. Three primer pairs were found to also amplify PCR products from three additional fiddler crab species (U. dampieri, U. flammula and U. seismella), and will prove useful for phylogeography and behavioural ecology of fiddler crabs.

KW - Fiddler crab

KW - Microsatellite

KW - Paternity

KW - Polymorphism

KW - Uca

UR - http://www.scopus.com/inward/record.url?scp=66549104632&partnerID=8YFLogxK

U2 - 10.1651/08-3049R.1

DO - 10.1651/08-3049R.1

M3 - Article

SN - 0278-0372

VL - 29

SP - 273

EP - 274

JO - Journal of Crustacean Biology

JF - Journal of Crustacean Biology

IS - 2

ER -

Polymorphic Microsatellite loci for paternity analysis in the fiddler crab Uca mjoebergi

Abstract

Access to Document

Other files and links

Fingerprint

Cite this