Population structure of the rice sheath blight pathogen Rhizoctonia solani AG-1 IA from India

Celeste C. Linde; Marcello Zala; R. S.David Paulraj; Bruce A. McDonald; Sam S. Gnanamanickam

doi:10.1007/s10658-005-1753-3

Population structure of the rice sheath blight pathogen Rhizoctonia solani AG-1 IA from India

Celeste C. Linde^*, Marcello Zala, R. S.David Paulraj, Bruce A. McDonald, Sam S. Gnanamanickam

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

The population structure of Rhizoctonia solani AG-1 IA causing rice sheath blight from India was evaluated for 96 isolates using seven RFLP loci. Nineteen of the isolates did not hybridise to R. solani AG-1 IA RFLP probes and rDNA analyses subsequently confirmed that they were either Ceratobasidium oryzae-sativae isolates or another Rhizoctonia sp. The population structure of the remaining 77 R. solani AG-1 IA Indian isolates was similar to that of a previously characterized Texas population. Clonal dispersal of R. solani AG-1 IA in India was moderate within fields and no clones were shared among field populations. Low levels of population subdivision and small genetic distances among populations were consistent with high levels of gene flow. Frequent sexual reproduction was indicated by the fact that most populations were in Hardy-Weinberg equilibrium (HWE). The two loci (R68 and R111) that deviated significantly from HWE showed an excess of heterozygosity. Although Texas and Indian populations were geographically very distant, they exhibited only moderate population subdivision, with an F _ST value of 0.193.

Original language	English
Pages (from-to)	113-121
Number of pages	9
Journal	European Journal of Plant Pathology
Volume	112
Issue number	2
DOIs	https://doi.org/10.1007/s10658-005-1753-3
Publication status	Published - Jun 2005

Access to Document

10.1007/s10658-005-1753-3

Cite this

@article{772f3b4b7f374c84ab8450301de4fbc9,

title = "Population structure of the rice sheath blight pathogen Rhizoctonia solani AG-1 IA from India",

abstract = "The population structure of Rhizoctonia solani AG-1 IA causing rice sheath blight from India was evaluated for 96 isolates using seven RFLP loci. Nineteen of the isolates did not hybridise to R. solani AG-1 IA RFLP probes and rDNA analyses subsequently confirmed that they were either Ceratobasidium oryzae-sativae isolates or another Rhizoctonia sp. The population structure of the remaining 77 R. solani AG-1 IA Indian isolates was similar to that of a previously characterized Texas population. Clonal dispersal of R. solani AG-1 IA in India was moderate within fields and no clones were shared among field populations. Low levels of population subdivision and small genetic distances among populations were consistent with high levels of gene flow. Frequent sexual reproduction was indicated by the fact that most populations were in Hardy-Weinberg equilibrium (HWE). The two loci (R68 and R111) that deviated significantly from HWE showed an excess of heterozygosity. Although Texas and Indian populations were geographically very distant, they exhibited only moderate population subdivision, with an F ST value of 0.193.",

keywords = "Ceratobasidium oryzae-sativae, Excess heterozygosity, Population genetics, rDNA analyses",

author = "Linde, {Celeste C.} and Marcello Zala and Paulraj, {R. S.David} and McDonald, {Bruce A.} and Gnanamanickam, {Sam S.}",

year = "2005",

month = jun,

doi = "10.1007/s10658-005-1753-3",

language = "English",

volume = "112",

pages = "113--121",

journal = "European Journal of Plant Pathology",

issn = "0929-1873",

publisher = "Springer Netherlands",

number = "2",

}

TY - JOUR

T1 - Population structure of the rice sheath blight pathogen Rhizoctonia solani AG-1 IA from India

AU - Linde, Celeste C.

AU - Zala, Marcello

AU - Paulraj, R. S.David

AU - McDonald, Bruce A.

AU - Gnanamanickam, Sam S.

PY - 2005/6

Y1 - 2005/6

N2 - The population structure of Rhizoctonia solani AG-1 IA causing rice sheath blight from India was evaluated for 96 isolates using seven RFLP loci. Nineteen of the isolates did not hybridise to R. solani AG-1 IA RFLP probes and rDNA analyses subsequently confirmed that they were either Ceratobasidium oryzae-sativae isolates or another Rhizoctonia sp. The population structure of the remaining 77 R. solani AG-1 IA Indian isolates was similar to that of a previously characterized Texas population. Clonal dispersal of R. solani AG-1 IA in India was moderate within fields and no clones were shared among field populations. Low levels of population subdivision and small genetic distances among populations were consistent with high levels of gene flow. Frequent sexual reproduction was indicated by the fact that most populations were in Hardy-Weinberg equilibrium (HWE). The two loci (R68 and R111) that deviated significantly from HWE showed an excess of heterozygosity. Although Texas and Indian populations were geographically very distant, they exhibited only moderate population subdivision, with an F ST value of 0.193.

AB - The population structure of Rhizoctonia solani AG-1 IA causing rice sheath blight from India was evaluated for 96 isolates using seven RFLP loci. Nineteen of the isolates did not hybridise to R. solani AG-1 IA RFLP probes and rDNA analyses subsequently confirmed that they were either Ceratobasidium oryzae-sativae isolates or another Rhizoctonia sp. The population structure of the remaining 77 R. solani AG-1 IA Indian isolates was similar to that of a previously characterized Texas population. Clonal dispersal of R. solani AG-1 IA in India was moderate within fields and no clones were shared among field populations. Low levels of population subdivision and small genetic distances among populations were consistent with high levels of gene flow. Frequent sexual reproduction was indicated by the fact that most populations were in Hardy-Weinberg equilibrium (HWE). The two loci (R68 and R111) that deviated significantly from HWE showed an excess of heterozygosity. Although Texas and Indian populations were geographically very distant, they exhibited only moderate population subdivision, with an F ST value of 0.193.

KW - Ceratobasidium oryzae-sativae

KW - Excess heterozygosity

KW - Population genetics

KW - rDNA analyses

UR - http://www.scopus.com/inward/record.url?scp=21144457295&partnerID=8YFLogxK

U2 - 10.1007/s10658-005-1753-3

DO - 10.1007/s10658-005-1753-3

M3 - Article

SN - 0929-1873

VL - 112

SP - 113

EP - 121

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

IS - 2

ER -

Population structure of the rice sheath blight pathogen Rhizoctonia solani AG-1 IA from India

Abstract

Access to Document

Other files and links

Fingerprint

Cite this