Preventive effect of N-acetylcysteine in a mouse model of steroid resistant acute exacerbation of asthma

Parivash Eftekhari; Sohrab Hajizadeh; Mohammad Reza Raoufy; Mohammad Reza Masjedi; Ming Yang; Nicole Hansbro; Jing Jing Li; Paul S. Foster

Preventive effect of N-acetylcysteine in a mouse model of steroid resistant acute exacerbation of asthma

Parivash Eftekhari, Sohrab Hajizadeh^*, Mohammad Reza Raoufy, Mohammad Reza Masjedi, Ming Yang, Nicole Hansbro, Jing Jing Li, Paul S. Foster

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Oxidative stress appears to have an important role in glucocorticoid insensitivity, as a crucial problem in asthma therapy. We studied the preventive effect of antioxidant N-acetylcysteine (NAC) on the airway hyper-responsiveness (AHR) and the accumulation of inflammatory cells in the airways in an animal model of steroid resistant acute exacerbation of asthma. Systemically sensitized Balb/C mice were exposed to Ovalbumin aerosol on days 13, 14, 15 and 16, followed by intratracheal lipopolysaccharide (LPS) to induce acute exacerbation. NAC (intraperitoneal, 320 mg/kg 30 min before and 12 hours after each challenge) reduced hyperresponsiveness with/out dexamethasone. LPS application caused neutrophilia in bronchoalveolar lavage fluid (BALF) and eosinophil count was higher than respective control in BALF as well as neutrophils after dexamethasone treatment. NAC significantly decreased neutrophil and eosinophil count in BALF as well as inflammatory cytokines (IL-13 and IL-5).We concluded that addition of NAC to asthma therapy has beneficial preventive effects in an animal model of steroid resistant acute exacerbation of asthma.

Original language	English
Pages (from-to)	184-192
Number of pages	9
Journal	EXCLI Journal
Volume	12
Publication status	Published - 11 Mar 2013

Cite this

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title = "Preventive effect of N-acetylcysteine in a mouse model of steroid resistant acute exacerbation of asthma",

abstract = "Oxidative stress appears to have an important role in glucocorticoid insensitivity, as a crucial problem in asthma therapy. We studied the preventive effect of antioxidant N-acetylcysteine (NAC) on the airway hyper-responsiveness (AHR) and the accumulation of inflammatory cells in the airways in an animal model of steroid resistant acute exacerbation of asthma. Systemically sensitized Balb/C mice were exposed to Ovalbumin aerosol on days 13, 14, 15 and 16, followed by intratracheal lipopolysaccharide (LPS) to induce acute exacerbation. NAC (intraperitoneal, 320 mg/kg 30 min before and 12 hours after each challenge) reduced hyperresponsiveness with/out dexamethasone. LPS application caused neutrophilia in bronchoalveolar lavage fluid (BALF) and eosinophil count was higher than respective control in BALF as well as neutrophils after dexamethasone treatment. NAC significantly decreased neutrophil and eosinophil count in BALF as well as inflammatory cytokines (IL-13 and IL-5).We concluded that addition of NAC to asthma therapy has beneficial preventive effects in an animal model of steroid resistant acute exacerbation of asthma.",

keywords = "Acute exacerbation, Eosinophil, N-acetylcysteine, Neutrophil, Steroid resistant asthma",

author = "Parivash Eftekhari and Sohrab Hajizadeh and {Reza Raoufy}, Mohammad and {Reza Masjedi}, Mohammad and Ming Yang and Nicole Hansbro and Li, {Jing Jing} and Foster, {Paul S.}",

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AU - Eftekhari, Parivash

AU - Hajizadeh, Sohrab

AU - Reza Raoufy, Mohammad

AU - Reza Masjedi, Mohammad

AU - Yang, Ming

AU - Hansbro, Nicole

AU - Li, Jing Jing

AU - Foster, Paul S.

PY - 2013/3/11

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N2 - Oxidative stress appears to have an important role in glucocorticoid insensitivity, as a crucial problem in asthma therapy. We studied the preventive effect of antioxidant N-acetylcysteine (NAC) on the airway hyper-responsiveness (AHR) and the accumulation of inflammatory cells in the airways in an animal model of steroid resistant acute exacerbation of asthma. Systemically sensitized Balb/C mice were exposed to Ovalbumin aerosol on days 13, 14, 15 and 16, followed by intratracheal lipopolysaccharide (LPS) to induce acute exacerbation. NAC (intraperitoneal, 320 mg/kg 30 min before and 12 hours after each challenge) reduced hyperresponsiveness with/out dexamethasone. LPS application caused neutrophilia in bronchoalveolar lavage fluid (BALF) and eosinophil count was higher than respective control in BALF as well as neutrophils after dexamethasone treatment. NAC significantly decreased neutrophil and eosinophil count in BALF as well as inflammatory cytokines (IL-13 and IL-5).We concluded that addition of NAC to asthma therapy has beneficial preventive effects in an animal model of steroid resistant acute exacerbation of asthma.

AB - Oxidative stress appears to have an important role in glucocorticoid insensitivity, as a crucial problem in asthma therapy. We studied the preventive effect of antioxidant N-acetylcysteine (NAC) on the airway hyper-responsiveness (AHR) and the accumulation of inflammatory cells in the airways in an animal model of steroid resistant acute exacerbation of asthma. Systemically sensitized Balb/C mice were exposed to Ovalbumin aerosol on days 13, 14, 15 and 16, followed by intratracheal lipopolysaccharide (LPS) to induce acute exacerbation. NAC (intraperitoneal, 320 mg/kg 30 min before and 12 hours after each challenge) reduced hyperresponsiveness with/out dexamethasone. LPS application caused neutrophilia in bronchoalveolar lavage fluid (BALF) and eosinophil count was higher than respective control in BALF as well as neutrophils after dexamethasone treatment. NAC significantly decreased neutrophil and eosinophil count in BALF as well as inflammatory cytokines (IL-13 and IL-5).We concluded that addition of NAC to asthma therapy has beneficial preventive effects in an animal model of steroid resistant acute exacerbation of asthma.

KW - Acute exacerbation

KW - Eosinophil

KW - N-acetylcysteine

KW - Neutrophil

KW - Steroid resistant asthma

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M3 - Article

SN - 1611-2156

VL - 12

SP - 184

EP - 192

JO - EXCLI Journal

JF - EXCLI Journal

ER -

Preventive effect of N-acetylcysteine in a mouse model of steroid resistant acute exacerbation of asthma

Abstract

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