Primary Intestinal Fibroblasts: Isolation, Cultivation, and Maintenance

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    Abstract

    Intestinal fibroblasts maintain homeostasis and contribute to inflammatory responses and the development of cancer. Intestinal fibroblasts express pattern recognition receptors which can mount an immune response. Since intestinal fibroblasts interact with diverse immune and nonimmune cells, further insights into the biology of intestinal fibroblasts could expand our knowledge of the development, homeostasis, and pathophysiology of the intestine. Here, we describe a simple protocol for the isolation, cultivation, and maintenance of primary fibroblasts from the mouse colon. These cells express α-smooth muscle actin, a characteristic of specialized contractile fibroblasts called myofibroblasts. We also outline the use of these colonic fibroblasts for immunoblotting and immunofluorescence assays with or without stimulation with a growth factor.
    Original languageEnglish
    Title of host publicationInflammation and Cancer
    EditorsBrendan J. Jenkins
    Place of PublicationUSA
    PublisherHumana, New York, NY
    Pages327-335
    Volume1
    ISBN (Print)9781071633304
    DOIs
    Publication statusPublished - 2023

    Publication series

    Name
    Volume2691

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