Prime-boost strategies in DNA vaccines.

C. Jane Dale*, Scott Thomson, Robert De Rose, Charani Ranasinghe, C. Jill Medveczky, Joko Pamungkas, David B. Boyle, Ian A. Ramshaw, Stephen J. Kent

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

45 Citations (Scopus)

Abstract

Induction of HIV-specific T-cell responses by vaccines may facilitate efficient control of HIV replication. Plasmid DNA vaccines and recombinant fowlpox virus (rFPV) vaccines are promising HIV-1 vaccine candidates, although delivering either vaccine alone may be insufficient to induce sufficient T-cell responses. A consecutive immunization strategy, known as "prime-boost," involving priming with DNA and boosting with rFPV vaccines encoding multiple common HIV antigens, is used to induce broad and high-level T-cell immunity and ameliorate AIDS in macaques. This vaccine strategy is proceeding to clinical trials. This chapter describes the use of prime-boost vaccines to induce T-cell responses against HIV-1 and protective immunity against AIDS in macaques. Methods for the construction of the vaccines, the use of animal models, and the detection of immune responses are described.

Original languageEnglish
Pages (from-to)171-197
Number of pages27
JournalMethods in molecular medicine
Volume127
Publication statusPublished - 2006
Externally publishedYes

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