Primers for novel microsatellite markers in "fire-specialist" lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations

Annabel L. Smith; Michael G. Gardner; C. Michael Bull; Don A. Driscoll

doi:10.1007/s12686-010-9359-2

Primers for novel microsatellite markers in "fire-specialist" lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations

Annabel L. Smith, Michael G. Gardner, C. Michael Bull, Don A. Driscoll

Research output: Contribution to journal › Comment/debate › peer-review

6 Citations (Scopus)

Abstract

We developed 45 microsatellite markers for three lizard species with fire-related distributions: Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus (17, 12 and 16 markers respectively). To isolate microsatellites we used an enrichment technique for N. stellatus and next-generation sequencing for A. norrisi and C. atlas. Fluorescent tags were attached to primers during PCR for flexible genotyping. All loci were polymorphic with 2-24 alleles and expected heterozygosities of 0.043-0.927. These markers will facilitate studies of post-fire dispersal and recolonisation.

Original language	English
Pages (from-to)	345-350
Number of pages	6
Journal	Conservation Genetics Resources
Volume	3
Issue number	2
DOIs	https://doi.org/10.1007/s12686-010-9359-2
Publication status	Published - Apr 2011

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abstract = "We developed 45 microsatellite markers for three lizard species with fire-related distributions: Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus (17, 12 and 16 markers respectively). To isolate microsatellites we used an enrichment technique for N. stellatus and next-generation sequencing for A. norrisi and C. atlas. Fluorescent tags were attached to primers during PCR for flexible genotyping. All loci were polymorphic with 2-24 alleles and expected heterozygosities of 0.043-0.927. These markers will facilitate studies of post-fire dispersal and recolonisation.",

keywords = "Dispersal, Fire ecology, Gene flow, Population genetics, Rept",

author = "Smith, {Annabel L.} and Gardner, {Michael G.} and Bull, {C. Michael} and Driscoll, {Don A.}",

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Primers for novel microsatellite markers in "fire-specialist" lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations. / Smith, Annabel L.; Gardner, Michael G.; Bull, C. Michael et al.
In: Conservation Genetics Resources, Vol. 3, No. 2, 04.2011, p. 345-350.

Research output: Contribution to journal › Comment/debate › peer-review

TY - JOUR

T1 - Primers for novel microsatellite markers in "fire-specialist" lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations

AU - Smith, Annabel L.

AU - Gardner, Michael G.

AU - Bull, C. Michael

AU - Driscoll, Don A.

PY - 2011/4

Y1 - 2011/4

N2 - We developed 45 microsatellite markers for three lizard species with fire-related distributions: Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus (17, 12 and 16 markers respectively). To isolate microsatellites we used an enrichment technique for N. stellatus and next-generation sequencing for A. norrisi and C. atlas. Fluorescent tags were attached to primers during PCR for flexible genotyping. All loci were polymorphic with 2-24 alleles and expected heterozygosities of 0.043-0.927. These markers will facilitate studies of post-fire dispersal and recolonisation.

AB - We developed 45 microsatellite markers for three lizard species with fire-related distributions: Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus (17, 12 and 16 markers respectively). To isolate microsatellites we used an enrichment technique for N. stellatus and next-generation sequencing for A. norrisi and C. atlas. Fluorescent tags were attached to primers during PCR for flexible genotyping. All loci were polymorphic with 2-24 alleles and expected heterozygosities of 0.043-0.927. These markers will facilitate studies of post-fire dispersal and recolonisation.

KW - Dispersal

KW - Fire ecology

KW - Gene flow

KW - Population genetics

KW - Rept

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U2 - 10.1007/s12686-010-9359-2

DO - 10.1007/s12686-010-9359-2

M3 - Comment/debate

SN - 1877-7252

VL - 3

SP - 345

EP - 350

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 2

ER -

Primers for novel microsatellite markers in "fire-specialist" lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations

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