Probing for pH-regulated proteins in Sinorhizobium medicae using proteomic analysis

Wayne G. Reeve; Ravi P. Tiwari; Nelson Guerreiro; Janine Stubbs; Michael J. Dilworth; Andrew R. Glenn; Barry G. Rolfe; Michael A. Djordjevic; John G. Howieson

doi:10.1159/000078657

Probing for pH-regulated proteins in Sinorhizobium medicae using proteomic analysis

Wayne G. Reeve^*, Ravi P. Tiwari, Nelson Guerreiro, Janine Stubbs, Michael J. Dilworth, Andrew R. Glenn, Barry G. Rolfe, Michael A. Djordjevic, John G. Howieson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

To elucidate the mechanisms of pH response in an acid-tolerant Sinorhizobium medicae strain we have identifi ed acid-activated gene transcription and now complement this approach by using a proteomic analysis to identify the changes that occur following exposure to acidity. Protein profi les of persistently or transiently acid-stressed S. medicae cells were compared to those grown in pH neutral, buffered media. Fifty pH-regulated proteins were identified; N-terminal sequences for 15 of these were obtained using the Edman degradation. Transient acid exposure downregulated GlnA and GlnK and upregulated a hypothetical protein. Continuing acid exposure downregulated ClpP, an ABC transporter, a hypothetical protein, a lipoprotein, the Trp-like repressor WrbA1 and upregulated DegP, fructose bisphosphate aldolase, GroES, malate dehydrogenase and two hypothetical proteins. These findings implicate proteolytic, chaperone and transport processes as key components of pH response in S. medicae .

Original language	English
Pages (from-to)	140-147
Number of pages	8
Journal	Journal of Molecular Microbiology and Biotechnology
Volume	7
Issue number	3
DOIs	https://doi.org/10.1159/000078657
Publication status	Published - 2004

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@article{e82be2bd67164aaebfbc050497493ccb,

title = "Probing for pH-regulated proteins in Sinorhizobium medicae using proteomic analysis",

abstract = "To elucidate the mechanisms of pH response in an acid-tolerant Sinorhizobium medicae strain we have identifi ed acid-activated gene transcription and now complement this approach by using a proteomic analysis to identify the changes that occur following exposure to acidity. Protein profi les of persistently or transiently acid-stressed S. medicae cells were compared to those grown in pH neutral, buffered media. Fifty pH-regulated proteins were identified; N-terminal sequences for 15 of these were obtained using the Edman degradation. Transient acid exposure downregulated GlnA and GlnK and upregulated a hypothetical protein. Continuing acid exposure downregulated ClpP, an ABC transporter, a hypothetical protein, a lipoprotein, the Trp-like repressor WrbA1 and upregulated DegP, fructose bisphosphate aldolase, GroES, malate dehydrogenase and two hypothetical proteins. These findings implicate proteolytic, chaperone and transport processes as key components of pH response in S. medicae .",

keywords = "Acid response, Acidity, Low pH, Proteomic analysis, Sinorhizobium",

author = "Reeve, {Wayne G.} and Tiwari, {Ravi P.} and Nelson Guerreiro and Janine Stubbs and Dilworth, {Michael J.} and Glenn, {Andrew R.} and Rolfe, {Barry G.} and Djordjevic, {Michael A.} and Howieson, {John G.}",

year = "2004",

doi = "10.1159/000078657",

language = "English",

volume = "7",

pages = "140--147",

journal = "Journal of Molecular Microbiology and Biotechnology",

issn = "1464-1801",

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T1 - Probing for pH-regulated proteins in Sinorhizobium medicae using proteomic analysis

AU - Reeve, Wayne G.

AU - Tiwari, Ravi P.

AU - Guerreiro, Nelson

AU - Stubbs, Janine

AU - Dilworth, Michael J.

AU - Glenn, Andrew R.

AU - Rolfe, Barry G.

AU - Djordjevic, Michael A.

AU - Howieson, John G.

PY - 2004

Y1 - 2004

N2 - To elucidate the mechanisms of pH response in an acid-tolerant Sinorhizobium medicae strain we have identifi ed acid-activated gene transcription and now complement this approach by using a proteomic analysis to identify the changes that occur following exposure to acidity. Protein profi les of persistently or transiently acid-stressed S. medicae cells were compared to those grown in pH neutral, buffered media. Fifty pH-regulated proteins were identified; N-terminal sequences for 15 of these were obtained using the Edman degradation. Transient acid exposure downregulated GlnA and GlnK and upregulated a hypothetical protein. Continuing acid exposure downregulated ClpP, an ABC transporter, a hypothetical protein, a lipoprotein, the Trp-like repressor WrbA1 and upregulated DegP, fructose bisphosphate aldolase, GroES, malate dehydrogenase and two hypothetical proteins. These findings implicate proteolytic, chaperone and transport processes as key components of pH response in S. medicae .

AB - To elucidate the mechanisms of pH response in an acid-tolerant Sinorhizobium medicae strain we have identifi ed acid-activated gene transcription and now complement this approach by using a proteomic analysis to identify the changes that occur following exposure to acidity. Protein profi les of persistently or transiently acid-stressed S. medicae cells were compared to those grown in pH neutral, buffered media. Fifty pH-regulated proteins were identified; N-terminal sequences for 15 of these were obtained using the Edman degradation. Transient acid exposure downregulated GlnA and GlnK and upregulated a hypothetical protein. Continuing acid exposure downregulated ClpP, an ABC transporter, a hypothetical protein, a lipoprotein, the Trp-like repressor WrbA1 and upregulated DegP, fructose bisphosphate aldolase, GroES, malate dehydrogenase and two hypothetical proteins. These findings implicate proteolytic, chaperone and transport processes as key components of pH response in S. medicae .

KW - Acid response

KW - Acidity

KW - Low pH

KW - Proteomic analysis

KW - Sinorhizobium

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U2 - 10.1159/000078657

DO - 10.1159/000078657

M3 - Article

SN - 1464-1801

VL - 7

SP - 140

EP - 147

JO - Journal of Molecular Microbiology and Biotechnology

JF - Journal of Molecular Microbiology and Biotechnology

IS - 3

ER -

Probing for pH-regulated proteins in Sinorhizobium medicae using proteomic analysis

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