Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies

Ronald J. Jackson; David B. Boyle; Charani Ranasinghe

doi:10.1007/978-1-4939-0410-5_5

Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies

Ronald J. Jackson, David B. Boyle, Charani Ranasinghe

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Although recombinant DNA and recombinant viral vectors expressing HIV antigens have yielded positive outcomes in animal models, these vaccines have not been effectively translated to humans. Despite this, there is still a high level of optimism that poxviral-based vaccine strategies could offer the best hope for developing an effective vaccine against not only HIV-1 but also other chronic diseases where good-quality T and B cell immunity is needed for protection. In this chapter we discuss step by step (1) how recombinant poxviral vectors co-expressing HIV antigens and promising mucosal/systemic adjuvants (e.g., IL-13Rα2) are constructed, (2) how these vectors can be used in alternative heterologous prime-boost immunization strategies, (3) how systemic and mucosal samples are prepared for analysis, followed by (4) two immunological assays: multicolor intracellular cytokine staining and tetramer/homing maker analysis that are used to evaluate effective systemic and mucosal T cell immunity.

Original language	English
Pages (from-to)	61-90
Number of pages	30
Journal	Methods in Molecular Biology
Volume	1143
DOIs	https://doi.org/10.1007/978-1-4939-0410-5_5
Publication status	Published - 2014

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title = "Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies",

abstract = "Although recombinant DNA and recombinant viral vectors expressing HIV antigens have yielded positive outcomes in animal models, these vaccines have not been effectively translated to humans. Despite this, there is still a high level of optimism that poxviral-based vaccine strategies could offer the best hope for developing an effective vaccine against not only HIV-1 but also other chronic diseases where good-quality T and B cell immunity is needed for protection. In this chapter we discuss step by step (1) how recombinant poxviral vectors co-expressing HIV antigens and promising mucosal/systemic adjuvants (e.g., IL-13Rα2) are constructed, (2) how these vectors can be used in alternative heterologous prime-boost immunization strategies, (3) how systemic and mucosal samples are prepared for analysis, followed by (4) two immunological assays: multicolor intracellular cytokine staining and tetramer/homing maker analysis that are used to evaluate effective systemic and mucosal T cell immunity.",

keywords = "DNA vaccines, Fowlpox virus, Homing marker analysis, Intracellular cytokine staining, Mucosal/systemic cell preparation, Mucosal/systemic vaccination, Poxvirus vectors, Prime-boost immunization, T cell immunity, Tetramer analysis, Vaccinia virus",

author = "Jackson, {Ronald J.} and Boyle, {David B.} and Charani Ranasinghe",

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year = "2014",

doi = "10.1007/978-1-4939-0410-5_5",

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AU - Jackson, Ronald J.

AU - Boyle, David B.

AU - Ranasinghe, Charani

N1 - Publisher Copyright: © Springer Science+Business Media New York 2014.

PY - 2014

Y1 - 2014

N2 - Although recombinant DNA and recombinant viral vectors expressing HIV antigens have yielded positive outcomes in animal models, these vaccines have not been effectively translated to humans. Despite this, there is still a high level of optimism that poxviral-based vaccine strategies could offer the best hope for developing an effective vaccine against not only HIV-1 but also other chronic diseases where good-quality T and B cell immunity is needed for protection. In this chapter we discuss step by step (1) how recombinant poxviral vectors co-expressing HIV antigens and promising mucosal/systemic adjuvants (e.g., IL-13Rα2) are constructed, (2) how these vectors can be used in alternative heterologous prime-boost immunization strategies, (3) how systemic and mucosal samples are prepared for analysis, followed by (4) two immunological assays: multicolor intracellular cytokine staining and tetramer/homing maker analysis that are used to evaluate effective systemic and mucosal T cell immunity.

AB - Although recombinant DNA and recombinant viral vectors expressing HIV antigens have yielded positive outcomes in animal models, these vaccines have not been effectively translated to humans. Despite this, there is still a high level of optimism that poxviral-based vaccine strategies could offer the best hope for developing an effective vaccine against not only HIV-1 but also other chronic diseases where good-quality T and B cell immunity is needed for protection. In this chapter we discuss step by step (1) how recombinant poxviral vectors co-expressing HIV antigens and promising mucosal/systemic adjuvants (e.g., IL-13Rα2) are constructed, (2) how these vectors can be used in alternative heterologous prime-boost immunization strategies, (3) how systemic and mucosal samples are prepared for analysis, followed by (4) two immunological assays: multicolor intracellular cytokine staining and tetramer/homing maker analysis that are used to evaluate effective systemic and mucosal T cell immunity.

KW - DNA vaccines

KW - Fowlpox virus

KW - Homing marker analysis

KW - Intracellular cytokine staining

KW - Mucosal/systemic cell preparation

KW - Mucosal/systemic vaccination

KW - Poxvirus vectors

KW - Prime-boost immunization

KW - T cell immunity

KW - Tetramer analysis

KW - Vaccinia virus

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VL - 1143

SP - 61

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies

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