Protecting role of aunp conjugated insulin on piamater and granular region of mice brain damaged by PTZ induced seizure

Proteins and peptides play an important role in seizure prevention and neuroprotection and development of CNS. Acute increase in peripheral insulin level leads to higher CSF insulin, while chronic peripheral hyper-insulinemia down regulates insulin receptors in BBB, impairing insulin transport in the brain. Thus, increased insulin level in the circulatory pathway can affect the brain's normal activity. Nanoparticles are small enough to cross this BBB enhancing human cells' uptake but won't induce cell death. In this study, protecting ability of insulin and AuNP conjugated insulin was tested in PTZ induced seizure associated brain damages in mice. After specific duration mice were sacrificed by cervical dislocation. Brains were collected at once and were fixed with carnoy's fixatives. Brains' sections were made in rotary microtome at 5 μm thickness. Histological slides were observed under microscope. Piamater and granular region was more or less intact in insulin treated mice group and total prevention was observed in AuNP conjugated insulin administered animal model.