Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-l-phenylalanine

E. H. Abdelkader; A. Feintuch; X. Yao; L. A. Adams; L. Aurelio; B. Graham; D. Goldfarb; G. Otting

doi:10.1039/c5cc07121f

Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-l-phenylalanine

E. H. Abdelkader, A. Feintuch, X. Yao, L. A. Adams, L. Aurelio, B. Graham, D. Goldfarb^*, G. Otting

^*Corresponding author for this work

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66 Citations (Scopus)

Abstract

Quantitative cysteine-independent ligation of a Gd³⁺ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd³⁺-Gd³⁺ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd³⁺ ion relative to the protein can be predicted with high accuracy.

Original language	English
Pages (from-to)	15898-15901
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	88
DOIs	https://doi.org/10.1039/c5cc07121f
Publication status	Published - 2015

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abstract = "Quantitative cysteine-independent ligation of a Gd3+ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd3+-Gd3+ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd3+ ion relative to the protein can be predicted with high accuracy.",

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AU - Abdelkader, E. H.

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AU - Adams, L. A.

AU - Aurelio, L.

AU - Graham, B.

AU - Goldfarb, D.

AU - Otting, G.

PY - 2015

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AB - Quantitative cysteine-independent ligation of a Gd3+ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd3+-Gd3+ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd3+ ion relative to the protein can be predicted with high accuracy.

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Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-l-phenylalanine

Abstract

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