Protein NMR Resonance Assignment without Spectral Analysis: 5D SOlid-State Automated Projection SpectroscopY (SO-APSY)

Henry W. Orton; Jan Stanek; Tobias Schubeis; Dylan Foucaudeau; Claire Ollier; Adrian W. Draney; Tanguy Le Marchand; Diane Cala-De Paepe; Isabella C. Felli; Roberta Pierattelli; Sebastian Hiller; Wolfgang Bermel; Guido Pintacuda

doi:10.1002/anie.201912211

Protein NMR Resonance Assignment without Spectral Analysis: 5D SOlid-State Automated Projection SpectroscopY (SO-APSY)

Henry W. Orton, Jan Stanek, Tobias Schubeis, Dylan Foucaudeau, Claire Ollier, Adrian W. Draney, Tanguy Le Marchand, Diane Cala-De Paepe, Isabella C. Felli, Roberta Pierattelli, Sebastian Hiller, Wolfgang Bermel, Guido Pintacuda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Narrow proton signals, high sensitivity, and efficient coherence transfers provided by fast magic-angle spinning at high magnetic fields make automated projection spectroscopy feasible for the solid-state NMR analysis of proteins. We present the first ultrahigh dimensional implementation of this approach, where 5D peak lists are reconstructed from a number of 2D projections for protein samples of different molecular sizes and aggregation states, which show limited dispersion of chemical shifts or inhomogeneous broadenings. The resulting datasets are particularly suitable to automated analysis and yield rapid and unbiased assignments of backbone resonances.

Original language	English
Pages (from-to)	2380-2384
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	59
Issue number	6
DOIs	https://doi.org/10.1002/anie.201912211
Publication status	Published - 3 Feb 2020

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10.1002/anie.201912211

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Orton, H. W., Stanek, J., Schubeis, T., Foucaudeau, D., Ollier, C., Draney, A. W., Le Marchand, T., Cala-De Paepe, D., Felli, I. C., Pierattelli, R., Hiller, S., Bermel, W., & Pintacuda, G. (2020). Protein NMR Resonance Assignment without Spectral Analysis: 5D SOlid-State Automated Projection SpectroscopY (SO-APSY). Angewandte Chemie - International Edition, 59(6), 2380-2384. https://doi.org/10.1002/anie.201912211

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title = "Protein NMR Resonance Assignment without Spectral Analysis: 5D SOlid-State Automated Projection SpectroscopY (SO-APSY)",

abstract = "Narrow proton signals, high sensitivity, and efficient coherence transfers provided by fast magic-angle spinning at high magnetic fields make automated projection spectroscopy feasible for the solid-state NMR analysis of proteins. We present the first ultrahigh dimensional implementation of this approach, where 5D peak lists are reconstructed from a number of 2D projections for protein samples of different molecular sizes and aggregation states, which show limited dispersion of chemical shifts or inhomogeneous broadenings. The resulting datasets are particularly suitable to automated analysis and yield rapid and unbiased assignments of backbone resonances.",

keywords = "NMR spectroscopy, automation, projection spectroscopy, proton detection, solid-state structures",

author = "Orton, {Henry W.} and Jan Stanek and Tobias Schubeis and Dylan Foucaudeau and Claire Ollier and Draney, {Adrian W.} and {Le Marchand}, Tanguy and {Cala-De Paepe}, Diane and Felli, {Isabella C.} and Roberta Pierattelli and Sebastian Hiller and Wolfgang Bermel and Guido Pintacuda",

note = "Publisher Copyright: {\textcopyright} 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2020",

month = feb,

day = "3",

doi = "10.1002/anie.201912211",

language = "English",

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Orton, HW, Stanek, J, Schubeis, T, Foucaudeau, D, Ollier, C, Draney, AW, Le Marchand, T, Cala-De Paepe, D, Felli, IC, Pierattelli, R, Hiller, S, Bermel, W & Pintacuda, G 2020, 'Protein NMR Resonance Assignment without Spectral Analysis: 5D SOlid-State Automated Projection SpectroscopY (SO-APSY)', Angewandte Chemie - International Edition, vol. 59, no. 6, pp. 2380-2384. https://doi.org/10.1002/anie.201912211

TY - JOUR

T1 - Protein NMR Resonance Assignment without Spectral Analysis

T2 - 5D SOlid-State Automated Projection SpectroscopY (SO-APSY)

AU - Orton, Henry W.

AU - Stanek, Jan

AU - Schubeis, Tobias

AU - Foucaudeau, Dylan

AU - Ollier, Claire

AU - Draney, Adrian W.

AU - Le Marchand, Tanguy

AU - Cala-De Paepe, Diane

AU - Felli, Isabella C.

AU - Pierattelli, Roberta

AU - Hiller, Sebastian

AU - Bermel, Wolfgang

AU - Pintacuda, Guido

PY - 2020/2/3

Y1 - 2020/2/3

N2 - Narrow proton signals, high sensitivity, and efficient coherence transfers provided by fast magic-angle spinning at high magnetic fields make automated projection spectroscopy feasible for the solid-state NMR analysis of proteins. We present the first ultrahigh dimensional implementation of this approach, where 5D peak lists are reconstructed from a number of 2D projections for protein samples of different molecular sizes and aggregation states, which show limited dispersion of chemical shifts or inhomogeneous broadenings. The resulting datasets are particularly suitable to automated analysis and yield rapid and unbiased assignments of backbone resonances.

AB - Narrow proton signals, high sensitivity, and efficient coherence transfers provided by fast magic-angle spinning at high magnetic fields make automated projection spectroscopy feasible for the solid-state NMR analysis of proteins. We present the first ultrahigh dimensional implementation of this approach, where 5D peak lists are reconstructed from a number of 2D projections for protein samples of different molecular sizes and aggregation states, which show limited dispersion of chemical shifts or inhomogeneous broadenings. The resulting datasets are particularly suitable to automated analysis and yield rapid and unbiased assignments of backbone resonances.

KW - NMR spectroscopy

KW - automation

KW - projection spectroscopy

KW - proton detection

KW - solid-state structures

UR - http://www.scopus.com/inward/record.url?scp=85076770518&partnerID=8YFLogxK

U2 - 10.1002/anie.201912211

DO - 10.1002/anie.201912211

M3 - Article

SN - 1433-7851

VL - 59

SP - 2380

EP - 2384

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 6

ER -

Protein NMR Resonance Assignment without Spectral Analysis: 5D SOlid-State Automated Projection SpectroscopY (SO-APSY)

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