Protein signatures correspond to survival outcomes of AJCC stage III melanoma patients

Swetlana Mactier*, Kimberley L. Kaufman, Penghao Wang, Ben Crossett, Gulietta M. Pupo, Philippa L. Kohnke, John F. Thompson, Richard A. Scolyer, Jean Y. Yang, Graham J. Mann, Richard I. Christopherson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Outcomes for melanoma patients with stage III disease differ widely even within the same subcategory. Molecular signatures that more accurately predict prognosis are needed to stratify patients according to risk. Proteomic analyses were used to identify differentially abundant proteins in extracts of surgically excised samples from patients with stage IIIc melanoma lymph node metastases. Analysis of samples from patients with poor (n = 14, <1 yr) and good (n = 19, >4 yr) survival outcomes identified 84 proteins that were differentially abundant between prognostic groups. Subsequent selected reaction monitoring analysis verified 21 proteins as potential biomarkers for survival. Poor prognosis patients are characterized by increased levels of proteins involved in protein metabolism, nucleic acid metabolism, angiogenesis, deregulation of cellular energetics and methylation processes, and decreased levels of proteins involved in apoptosis and immune response. These proteins are able to classify stage IIIc patients into prognostic subgroups (P < 0.02). This is the first report of potential prognostic markers from stage III melanoma using proteomic analyses. Validation of these protein markers in larger patient cohorts should define protein signatures that enable better stratification of stage III melanoma patients.

Original languageEnglish
Pages (from-to)1106-1116
Number of pages11
JournalPigment Cell and Melanoma Research
Issue number6
Publication statusPublished - 1 Nov 2014
Externally publishedYes


Dive into the research topics of 'Protein signatures correspond to survival outcomes of AJCC stage III melanoma patients'. Together they form a unique fingerprint.

Cite this