Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling.

Dmitri Mouradov; Gordon King; Ian L. Ross; Jade K. Forwood; David A. Hume; Andrea Sinz; Jennifer L. Martin; Bostjan Kobe; Thomas Huber

doi:10.1007/978-1-60327-058-8_31

Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling.

Dmitri Mouradov^*, Gordon King, Ian L. Ross, Jade K. Forwood, David A. Hume, Andrea Sinz, Jennifer L. Martin, Bostjan Kobe, Thomas Huber

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Cross-linking in combination with mass spectrometry can be used as a tool for structural modeling of protein complexes and multidomain proteins. Although cross-links represent only weak structural constraints, the combination of a limited set of experimental cross-links with molecular docking/modeling is often sufficient to determine the structure of a protein complex or multidomain protein at low resolution.

Original language	English
Pages (from-to)	459-474
Number of pages	16
Journal	Methods in Molecular Biology
Volume	426
DOIs	https://doi.org/10.1007/978-1-60327-058-8_31
Publication status	Published - 2008
Externally published	Yes

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title = "Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling.",

abstract = "Cross-linking in combination with mass spectrometry can be used as a tool for structural modeling of protein complexes and multidomain proteins. Although cross-links represent only weak structural constraints, the combination of a limited set of experimental cross-links with molecular docking/modeling is often sufficient to determine the structure of a protein complex or multidomain protein at low resolution.",

author = "Dmitri Mouradov and Gordon King and Ross, \{Ian L.\} and Forwood, \{Jade K.\} and Hume, \{David A.\} and Andrea Sinz and Martin, \{Jennifer L.\} and Bostjan Kobe and Thomas Huber",

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AU - Mouradov, Dmitri

AU - King, Gordon

AU - Ross, Ian L.

AU - Forwood, Jade K.

AU - Hume, David A.

AU - Sinz, Andrea

AU - Martin, Jennifer L.

AU - Kobe, Bostjan

AU - Huber, Thomas

PY - 2008

Y1 - 2008

N2 - Cross-linking in combination with mass spectrometry can be used as a tool for structural modeling of protein complexes and multidomain proteins. Although cross-links represent only weak structural constraints, the combination of a limited set of experimental cross-links with molecular docking/modeling is often sufficient to determine the structure of a protein complex or multidomain protein at low resolution.

AB - Cross-linking in combination with mass spectrometry can be used as a tool for structural modeling of protein complexes and multidomain proteins. Although cross-links represent only weak structural constraints, the combination of a limited set of experimental cross-links with molecular docking/modeling is often sufficient to determine the structure of a protein complex or multidomain protein at low resolution.

UR - https://www.scopus.com/pages/publications/49649097784

U2 - 10.1007/978-1-60327-058-8_31

DO - 10.1007/978-1-60327-058-8_31

M3 - Article

C2 - 18542884

AN - SCOPUS:49649097784

SN - 1064-3745

VL - 426

SP - 459

EP - 474

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling.

Abstract

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