Proteomic dissection of DNA polymerization

Jennifer L. Beck*, Thitima Urathamakul, Stephen J. Watt, Margaret M. Sheil, Patrick M. Schaeffer, Nicholas E. Dixon

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    11 Citations (Scopus)


    DNA polymerases replicate the genome by associating with a range of other proteins that enable rapid, high-fidelity copying of DNA. This complex of proteins and nucleic acids is termed the replisome. Proteins of the replisome must interact with other networks of proteins, such as those involved in DNA repair. Many of the proteins involved in DNA polymerization and the accessory proteins are known, but the array of proteins they interact with, and the spatial and temporal arrangement of these interactions, are current research topics. Mass spectrometry is a technique that can be used to identify the sites of these interactions and to determine the precise stoichiometries of binding partners in a functional complex. A complete understanding of the macromolecular interactions involved in DNA replication and repair may lead to discovery of new targets for antibiotics against bacteria and biomarkers for diagnosis of diseases, such as cancer, in humans.

    Original languageEnglish
    Pages (from-to)197-211
    Number of pages15
    JournalExpert Review of Proteomics
    Issue number2
    Publication statusPublished - 2006


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