Abstract
It was recently demonstrated that the yeast homologue of phosphatidylinositol 4-kinaseβ PIK1 is directly associated with frq1, the yeast homologue of mammalian neuronal calcium sensor-1 (NCS-1) (Hendricks et al., [1999] Nat. Cell Biol. 1:234-241). This was a novel finding and suggests that a calcium binding protein activates and regulates PtdIns 4-kinaseβ. This finding had not been shown in mammalian cells and both PtdIns 4-kinaseβ and NCS-1 have been shown to have important roles in the regulation of exocytotic release associated with neurotransmission. The aims of this study were to determine if PtdIns 4-kinaseβ and NCS-1 directly associate in mammalian neural tissues. We show that the immunostaining pattern for PtdIns 4-kinaseβ and NCS-1 is co-localized throughout the neurites of newborn cultured dorsal root ganglia (DRG) neurons but not in E13 DRG neurons. We then provide biochemical evidence that PtdIns 4-kinaseβ may not be in physical association with NCS-1 in mammalian nervous tissue unlike that previously reported in yeast. (C) 2000 Wiley-Liss, Inc.
Original language | English |
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Pages (from-to) | 216-224 |
Number of pages | 9 |
Journal | Journal of Neuroscience Research |
Volume | 62 |
Issue number | 2 |
DOIs | |
Publication status | Published - 15 Oct 2000 |