Quantitative urinary excretion of unmetabolised N(τ)-[Me-1C]methylhistidine by the common ringtail possum (Pseudocheirus peregrinus) marsupialia

Shelley Watson; William J. Foley; Stuart McLean; Susan Brandon; Noel W. Davies

doi:10.1016/0300-9629(96)00001-1

Quantitative urinary excretion of unmetabolised N(τ)-[Me^-1C]methylhistidine by the common ringtail possum (Pseudocheirus peregrinus) marsupialia

Shelley Watson^*, William J. Foley, Stuart McLean, Susan Brandon, Noel W. Davies

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Six common ringtail possums (Pseudocheirus peregrinus) were intravenously injected with a standard dose of radioactive 3-Methylhistidine (N(τ)-[Me-¹⁴C]MeH). The dose was rapidly and quantitatively excreted by the possums. More than 90% of radioactivity was recovered within 3 days. Thin layer chromatography and mass spectroscopy showed that 97% of recovered radioactivity was associated with unmetabolised N(τ)-[Me¹⁴C]MeH. These data satisfy two key requirements for the validity of urinary 3-Methylhistidine (N(τ-)3MeH) excretion as an index of muscle protein catabolism.

Original language	English
Pages (from-to)	53-55
Number of pages	3
Journal	Comparative Biochemistry and Physiology - A Physiology
Volume	115
Issue number	1
DOIs	https://doi.org/10.1016/0300-9629(96)00001-1
Publication status	Published - Sept 1996
Externally published	Yes

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10.1016/0300-9629(96)00001-1

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@article{0f8a6e53ba9b4a11b77b7ef576b1da9b,

title = "Quantitative urinary excretion of unmetabolised N(τ)-[Me-1C]methylhistidine by the common ringtail possum (Pseudocheirus peregrinus) marsupialia",

abstract = "Six common ringtail possums (Pseudocheirus peregrinus) were intravenously injected with a standard dose of radioactive 3-Methylhistidine (N(τ)-[Me-14C]MeH). The dose was rapidly and quantitatively excreted by the possums. More than 90\% of radioactivity was recovered within 3 days. Thin layer chromatography and mass spectroscopy showed that 97\% of recovered radioactivity was associated with unmetabolised N(τ)-[Me14C]MeH. These data satisfy two key requirements for the validity of urinary 3-Methylhistidine (N(τ-)3MeH) excretion as an index of muscle protein catabolism.",

keywords = "3-methylhistidine, arboreal folivore, common ringtail possum, eucalyptus, marsupial, muscle protein catabolism, N(τ)-[Me-C]MeH, Pseudocheirus peregrinus",

author = "Shelley Watson and Foley, \{William J.\} and Stuart McLean and Susan Brandon and Davies, \{Noel W.\}",

year = "1996",

month = sep,

doi = "10.1016/0300-9629(96)00001-1",

language = "English",

volume = "115",

pages = "53--55",

journal = "Comparative Biochemistry and Physiology - A Physiology",

issn = "0300-9629",

publisher = "Elsevier Inc.",

number = "1",

}

Quantitative urinary excretion of unmetabolised N(τ)-[Me^-1C]methylhistidine by the common ringtail possum (Pseudocheirus peregrinus) marsupialia. / Watson, Shelley; Foley, William J.; McLean, Stuart et al.
In: Comparative Biochemistry and Physiology - A Physiology, Vol. 115, No. 1, 09.1996, p. 53-55.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Quantitative urinary excretion of unmetabolised N(τ)-[Me-1C]methylhistidine by the common ringtail possum (Pseudocheirus peregrinus) marsupialia

AU - Watson, Shelley

AU - Foley, William J.

AU - McLean, Stuart

AU - Brandon, Susan

AU - Davies, Noel W.

PY - 1996/9

Y1 - 1996/9

N2 - Six common ringtail possums (Pseudocheirus peregrinus) were intravenously injected with a standard dose of radioactive 3-Methylhistidine (N(τ)-[Me-14C]MeH). The dose was rapidly and quantitatively excreted by the possums. More than 90% of radioactivity was recovered within 3 days. Thin layer chromatography and mass spectroscopy showed that 97% of recovered radioactivity was associated with unmetabolised N(τ)-[Me14C]MeH. These data satisfy two key requirements for the validity of urinary 3-Methylhistidine (N(τ-)3MeH) excretion as an index of muscle protein catabolism.

AB - Six common ringtail possums (Pseudocheirus peregrinus) were intravenously injected with a standard dose of radioactive 3-Methylhistidine (N(τ)-[Me-14C]MeH). The dose was rapidly and quantitatively excreted by the possums. More than 90% of radioactivity was recovered within 3 days. Thin layer chromatography and mass spectroscopy showed that 97% of recovered radioactivity was associated with unmetabolised N(τ)-[Me14C]MeH. These data satisfy two key requirements for the validity of urinary 3-Methylhistidine (N(τ-)3MeH) excretion as an index of muscle protein catabolism.

KW - 3-methylhistidine

KW - arboreal folivore

KW - common ringtail possum

KW - eucalyptus

KW - marsupial

KW - muscle protein catabolism

KW - N(τ)-[Me-C]MeH

KW - Pseudocheirus peregrinus

UR - https://www.scopus.com/pages/publications/0030248237

U2 - 10.1016/0300-9629(96)00001-1

DO - 10.1016/0300-9629(96)00001-1

M3 - Article

C2 - 8858839

AN - SCOPUS:0030248237

SN - 0300-9629

VL - 115

SP - 53

EP - 55

JO - Comparative Biochemistry and Physiology - A Physiology

JF - Comparative Biochemistry and Physiology - A Physiology

IS - 1

ER -

Quantitative urinary excretion of unmetabolised N(τ)-[Me^-1C]methylhistidine by the common ringtail possum (Pseudocheirus peregrinus) marsupialia

Abstract

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