Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules

Kate S. Campbell; Carolyn T. Dillon; Suzanne V. Smith; Margaret M. Harding

doi:10.1016/j.poly.2006.07.004

Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules

Kate S. Campbell, Carolyn T. Dillon, Suzanne V. Smith^*, Margaret M. Harding

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Neutron irradiation of Cp₂MoCl₂ for 24 h afforded the radiotracer Cp₂⁹⁹MoCl₂ which was characterised by UV-Vis spectroscopy and thin layer chromatography. Binding experiments with the thiol containing protein human serum albumin (HSA) or calf thymus DNA, were monitored for ⁹⁹Mo using a gamma counter. Under the conditions investigated, molar ratios of binding of 0.2:1 (Cp₂MoCl₂:DNA) and 9.4:1 (Cp₂MoCl₂:HSA) were calculated. The results are consistent with in vitro coordination studies that have shown strong preferential interaction of Cp₂MoCl₂ with thiols versus other donor sites in biomolecules including DNA.

Original language	English
Pages (from-to)	456-459
Number of pages	4
Journal	Polyhedron
Volume	26
Issue number	2
DOIs	https://doi.org/10.1016/j.poly.2006.07.004
Publication status	Published - 22 Jan 2007
Externally published	Yes

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title = "Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules",

abstract = "Neutron irradiation of Cp2MoCl2 for 24 h afforded the radiotracer Cp299MoCl2 which was characterised by UV-Vis spectroscopy and thin layer chromatography. Binding experiments with the thiol containing protein human serum albumin (HSA) or calf thymus DNA, were monitored for 99Mo using a gamma counter. Under the conditions investigated, molar ratios of binding of 0.2:1 (Cp2MoCl2:DNA) and 9.4:1 (Cp2MoCl2:HSA) were calculated. The results are consistent with in vitro coordination studies that have shown strong preferential interaction of Cp2MoCl2 with thiols versus other donor sites in biomolecules including DNA.",

keywords = "Antitumor metallocene, DNA, Genotoxicity, Human serum albumin, Mo-radiotracer, Molybdocene dichloride",

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year = "2007",

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T1 - Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules

AU - Campbell, Kate S.

AU - Dillon, Carolyn T.

AU - Smith, Suzanne V.

AU - Harding, Margaret M.

PY - 2007/1/22

Y1 - 2007/1/22

N2 - Neutron irradiation of Cp2MoCl2 for 24 h afforded the radiotracer Cp299MoCl2 which was characterised by UV-Vis spectroscopy and thin layer chromatography. Binding experiments with the thiol containing protein human serum albumin (HSA) or calf thymus DNA, were monitored for 99Mo using a gamma counter. Under the conditions investigated, molar ratios of binding of 0.2:1 (Cp2MoCl2:DNA) and 9.4:1 (Cp2MoCl2:HSA) were calculated. The results are consistent with in vitro coordination studies that have shown strong preferential interaction of Cp2MoCl2 with thiols versus other donor sites in biomolecules including DNA.

AB - Neutron irradiation of Cp2MoCl2 for 24 h afforded the radiotracer Cp299MoCl2 which was characterised by UV-Vis spectroscopy and thin layer chromatography. Binding experiments with the thiol containing protein human serum albumin (HSA) or calf thymus DNA, were monitored for 99Mo using a gamma counter. Under the conditions investigated, molar ratios of binding of 0.2:1 (Cp2MoCl2:DNA) and 9.4:1 (Cp2MoCl2:HSA) were calculated. The results are consistent with in vitro coordination studies that have shown strong preferential interaction of Cp2MoCl2 with thiols versus other donor sites in biomolecules including DNA.

KW - Antitumor metallocene

KW - DNA

KW - Genotoxicity

KW - Human serum albumin

KW - Mo-radiotracer

KW - Molybdocene dichloride

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DO - 10.1016/j.poly.2006.07.004

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SP - 456

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JO - Polyhedron

JF - Polyhedron

IS - 2

ER -

Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules

Abstract

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