Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules

Kate S. Campbell; Carolyn T. Dillon; Suzanne V. Smith; Margaret M. Harding

doi:10.1016/j.poly.2006.07.004

Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules

Kate S. Campbell, Carolyn T. Dillon, Suzanne V. Smith^*, Margaret M. Harding

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Neutron irradiation of Cp₂MoCl₂ for 24 h afforded the radiotracer Cp₂⁹⁹MoCl₂ which was characterised by UV-Vis spectroscopy and thin layer chromatography. Binding experiments with the thiol containing protein human serum albumin (HSA) or calf thymus DNA, were monitored for ⁹⁹Mo using a gamma counter. Under the conditions investigated, molar ratios of binding of 0.2:1 (Cp₂MoCl₂:DNA) and 9.4:1 (Cp₂MoCl₂:HSA) were calculated. The results are consistent with in vitro coordination studies that have shown strong preferential interaction of Cp₂MoCl₂ with thiols versus other donor sites in biomolecules including DNA.

Original language	English
Pages (from-to)	456-459
Number of pages	4
Journal	Polyhedron
Volume	26
Issue number	2
DOIs	https://doi.org/10.1016/j.poly.2006.07.004
Publication status	Published - 22 Jan 2007
Externally published	Yes

Access to Document

10.1016/j.poly.2006.07.004

Cite this

@article{01cde51c4c284423a1ce595f802b46bf,

title = "Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules",

abstract = "Neutron irradiation of Cp2MoCl2 for 24 h afforded the radiotracer Cp299MoCl2 which was characterised by UV-Vis spectroscopy and thin layer chromatography. Binding experiments with the thiol containing protein human serum albumin (HSA) or calf thymus DNA, were monitored for 99Mo using a gamma counter. Under the conditions investigated, molar ratios of binding of 0.2:1 (Cp2MoCl2:DNA) and 9.4:1 (Cp2MoCl2:HSA) were calculated. The results are consistent with in vitro coordination studies that have shown strong preferential interaction of Cp2MoCl2 with thiols versus other donor sites in biomolecules including DNA.",

keywords = "Antitumor metallocene, DNA, Genotoxicity, Human serum albumin, Mo-radiotracer, Molybdocene dichloride",

author = "Campbell, \{Kate S.\} and Dillon, \{Carolyn T.\} and Smith, \{Suzanne V.\} and Harding, \{Margaret M.\}",

year = "2007",

month = jan,

day = "22",

doi = "10.1016/j.poly.2006.07.004",

language = "English",

volume = "26",

pages = "456--459",

journal = "Polyhedron",

issn = "0277-5387",

publisher = "Elsevier Ltd.",

number = "2",

}

TY - JOUR

T1 - Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules

AU - Campbell, Kate S.

AU - Dillon, Carolyn T.

AU - Smith, Suzanne V.

AU - Harding, Margaret M.

PY - 2007/1/22

Y1 - 2007/1/22

N2 - Neutron irradiation of Cp2MoCl2 for 24 h afforded the radiotracer Cp299MoCl2 which was characterised by UV-Vis spectroscopy and thin layer chromatography. Binding experiments with the thiol containing protein human serum albumin (HSA) or calf thymus DNA, were monitored for 99Mo using a gamma counter. Under the conditions investigated, molar ratios of binding of 0.2:1 (Cp2MoCl2:DNA) and 9.4:1 (Cp2MoCl2:HSA) were calculated. The results are consistent with in vitro coordination studies that have shown strong preferential interaction of Cp2MoCl2 with thiols versus other donor sites in biomolecules including DNA.

AB - Neutron irradiation of Cp2MoCl2 for 24 h afforded the radiotracer Cp299MoCl2 which was characterised by UV-Vis spectroscopy and thin layer chromatography. Binding experiments with the thiol containing protein human serum albumin (HSA) or calf thymus DNA, were monitored for 99Mo using a gamma counter. Under the conditions investigated, molar ratios of binding of 0.2:1 (Cp2MoCl2:DNA) and 9.4:1 (Cp2MoCl2:HSA) were calculated. The results are consistent with in vitro coordination studies that have shown strong preferential interaction of Cp2MoCl2 with thiols versus other donor sites in biomolecules including DNA.

KW - Antitumor metallocene

KW - DNA

KW - Genotoxicity

KW - Human serum albumin

KW - Mo-radiotracer

KW - Molybdocene dichloride

UR - https://www.scopus.com/pages/publications/33845867414

U2 - 10.1016/j.poly.2006.07.004

DO - 10.1016/j.poly.2006.07.004

M3 - Article

SN - 0277-5387

VL - 26

SP - 456

EP - 459

JO - Polyhedron

JF - Polyhedron

IS - 2

ER -

Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules

Abstract

Access to Document

Other files and links

Fingerprint

Cite this