TY - JOUR
T1 - Rapid and sensitive detection of nucleoprotein SARS-CoV-2 virus
T2 - SERS vs ELISA
AU - Fatkhutdinova, Landysh I.
AU - Babich, Ekaterina
AU - Boldyrev, Kirill
AU - Shipilovskikh, Sergei
AU - Terterev, Ivan
AU - Baranenko, Denis
AU - Redkov, Alexey
AU - Timin, Alexander
AU - Zyuzin, Mikhail V.
AU - Kivshar, Yuri
AU - Bogdanov, Andrey
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/12
Y1 - 2023/12
N2 - The recent pandemic calls for a rapid and cheap diagnosis of COVID-19 to impede the virus from spreading. The main clinically used techniques for diagnostics of virus diseases are PCR and ELISA, whereas it is well known that optical methods such as FTIR, Raman spectroscopy, and SERS are extremely precise, perspective, and also rapid. Here, we provide a comparative study of SERS and ELISA methods for the detection of SARS-CoV-2 nucleoproteins revealing and discussing the advantages and disadvantages of both these methods. We show that the standard ELISA method demonstrates the detection limit of about 50–100 pg/ml vs 1 pg/ml demonstrated by SERS with using silver nanoisland substrates. The detection time for ELISA is about 2 h being mainly defined by the requirements of incubation and washing, while SERS measurements take several seconds. We show that the SERS spectra allow distinguishing the features of antigens, antibodies, and even the features showing the bonding between them. We believe that the obtained results and the comparative analysis will be useful for developing cheap and rapid point-of-care diagnostics.
AB - The recent pandemic calls for a rapid and cheap diagnosis of COVID-19 to impede the virus from spreading. The main clinically used techniques for diagnostics of virus diseases are PCR and ELISA, whereas it is well known that optical methods such as FTIR, Raman spectroscopy, and SERS are extremely precise, perspective, and also rapid. Here, we provide a comparative study of SERS and ELISA methods for the detection of SARS-CoV-2 nucleoproteins revealing and discussing the advantages and disadvantages of both these methods. We show that the standard ELISA method demonstrates the detection limit of about 50–100 pg/ml vs 1 pg/ml demonstrated by SERS with using silver nanoisland substrates. The detection time for ELISA is about 2 h being mainly defined by the requirements of incubation and washing, while SERS measurements take several seconds. We show that the SERS spectra allow distinguishing the features of antigens, antibodies, and even the features showing the bonding between them. We believe that the obtained results and the comparative analysis will be useful for developing cheap and rapid point-of-care diagnostics.
KW - COVID-19
KW - ELISA
KW - FTIR
KW - SERS
UR - http://www.scopus.com/inward/record.url?scp=85166967056&partnerID=8YFLogxK
U2 - 10.1016/j.photonics.2023.101172
DO - 10.1016/j.photonics.2023.101172
M3 - Article
SN - 1569-4410
VL - 57
JO - Photonics and Nanostructures - Fundamentals and Applications
JF - Photonics and Nanostructures - Fundamentals and Applications
M1 - 101172
ER -