Rapid zero-trans kinetics of Cs+ exchange in human erythrocytes quantified by dissolution hyperpolarized 133Cs+ NMR spectroscopy

Philip W. Kuchel; Magnus Karlsson; Mathilde Hauge Lerche; Dmitry Shishmarev; Jan Henrik Ardenkjaer-Larsen

doi:10.1038/s41598-019-56250-z

Rapid zero-trans kinetics of Cs⁺ exchange in human erythrocytes quantified by dissolution hyperpolarized ¹³³Cs⁺ NMR spectroscopy

Philip W. Kuchel^*, Magnus Karlsson, Mathilde Hauge Lerche, Dmitry Shishmarev, Jan Henrik Ardenkjaer-Larsen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Transmembrane flux of Cs⁺ (a K⁺ congener) was measured in human red blood cells (RBCs; erythrocytes) on the 10-s time scale. This is the first report on dissolution dynamic nuclear polarization (dDNP) nuclear magnetic resonance (NMR) spectroscopy with this nuclide in mammalian cells. Four technical developments regularized sample delivery and led to high quality NMR spectra. Cation-free media with the Piezo1 (mechanosensitive cation channel) activator yoda1 maximized the extent of membrane transport. First-order rate constants describing the fluxes were estimated using a combination of statistical methods in Mathematica, including the Markov chain Monte Carlo (MCMC) algorithm. Fluxes were in the range 4–70 μmol Cs⁺ (L RBC)⁻¹ s⁻¹; these are smaller than for urea, but comparable to glucose. Methodology and analytical procedures developed will be applicable to transmembrane cation transport studies in the presence of additional Piezo1 effectors, to other cellular systems, and potentially in vivo.

Original language	English
Article number	19726
Journal	Scientific Reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-019-56250-z
Publication status	Published - 1 Dec 2019

Access to Document

10.1038/s41598-019-56250-z

Cite this

@article{34d35716923d4fc782db6fc945ab52ee,

title = "Rapid zero-trans kinetics of Cs+ exchange in human erythrocytes quantified by dissolution hyperpolarized 133Cs+ NMR spectroscopy",

abstract = "Transmembrane flux of Cs+ (a K+ congener) was measured in human red blood cells (RBCs; erythrocytes) on the 10-s time scale. This is the first report on dissolution dynamic nuclear polarization (dDNP) nuclear magnetic resonance (NMR) spectroscopy with this nuclide in mammalian cells. Four technical developments regularized sample delivery and led to high quality NMR spectra. Cation-free media with the Piezo1 (mechanosensitive cation channel) activator yoda1 maximized the extent of membrane transport. First-order rate constants describing the fluxes were estimated using a combination of statistical methods in Mathematica, including the Markov chain Monte Carlo (MCMC) algorithm. Fluxes were in the range 4–70 μmol Cs+ (L RBC)−1 s−1; these are smaller than for urea, but comparable to glucose. Methodology and analytical procedures developed will be applicable to transmembrane cation transport studies in the presence of additional Piezo1 effectors, to other cellular systems, and potentially in vivo.",

author = "Kuchel, {Philip W.} and Magnus Karlsson and Lerche, {Mathilde Hauge} and Dmitry Shishmarev and Ardenkjaer-Larsen, {Jan Henrik}",

note = "Publisher Copyright: {\textcopyright} 2019, The Author(s).",

year = "2019",

month = dec,

day = "1",

doi = "10.1038/s41598-019-56250-z",

language = "English",

volume = "9",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Rapid zero-trans kinetics of Cs+ exchange in human erythrocytes quantified by dissolution hyperpolarized 133Cs+ NMR spectroscopy

AU - Kuchel, Philip W.

AU - Karlsson, Magnus

AU - Lerche, Mathilde Hauge

AU - Shishmarev, Dmitry

AU - Ardenkjaer-Larsen, Jan Henrik

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Transmembrane flux of Cs+ (a K+ congener) was measured in human red blood cells (RBCs; erythrocytes) on the 10-s time scale. This is the first report on dissolution dynamic nuclear polarization (dDNP) nuclear magnetic resonance (NMR) spectroscopy with this nuclide in mammalian cells. Four technical developments regularized sample delivery and led to high quality NMR spectra. Cation-free media with the Piezo1 (mechanosensitive cation channel) activator yoda1 maximized the extent of membrane transport. First-order rate constants describing the fluxes were estimated using a combination of statistical methods in Mathematica, including the Markov chain Monte Carlo (MCMC) algorithm. Fluxes were in the range 4–70 μmol Cs+ (L RBC)−1 s−1; these are smaller than for urea, but comparable to glucose. Methodology and analytical procedures developed will be applicable to transmembrane cation transport studies in the presence of additional Piezo1 effectors, to other cellular systems, and potentially in vivo.

AB - Transmembrane flux of Cs+ (a K+ congener) was measured in human red blood cells (RBCs; erythrocytes) on the 10-s time scale. This is the first report on dissolution dynamic nuclear polarization (dDNP) nuclear magnetic resonance (NMR) spectroscopy with this nuclide in mammalian cells. Four technical developments regularized sample delivery and led to high quality NMR spectra. Cation-free media with the Piezo1 (mechanosensitive cation channel) activator yoda1 maximized the extent of membrane transport. First-order rate constants describing the fluxes were estimated using a combination of statistical methods in Mathematica, including the Markov chain Monte Carlo (MCMC) algorithm. Fluxes were in the range 4–70 μmol Cs+ (L RBC)−1 s−1; these are smaller than for urea, but comparable to glucose. Methodology and analytical procedures developed will be applicable to transmembrane cation transport studies in the presence of additional Piezo1 effectors, to other cellular systems, and potentially in vivo.

UR - http://www.scopus.com/inward/record.url?scp=85077038758&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-56250-z

DO - 10.1038/s41598-019-56250-z

M3 - Article

SN - 2045-2322

VL - 9

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 19726

ER -

Rapid zero-trans kinetics of Cs⁺ exchange in human erythrocytes quantified by dissolution hyperpolarized ¹³³Cs⁺ NMR spectroscopy

Abstract

Access to Document

Other files and links

Fingerprint

Cite this