RChIP-Seq: Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes

Tatiana A. Soboleva*, David J. Tremethick

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    1 Citation (Scopus)

    Abstract

    Chromatin is a dynamic macromolecular structure comprised of histones and a wealth of non-histone proteins. Recently, it has become clear that RNA is also an integral component of chromatin playing an important role in regulating its structure and function. Central to the understanding of RNA function is the ability to identify and genomically map interactions between chromatin components and RNA. Here, we describe a new method, RChIP-seq (RNA-associated-Chromatin-Immuno Precipitation followed by next-generation sequencing) that allows the identification of RNA species that are directly bound to specific components of chromatin in the mouse testis.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages169-184
    Number of pages16
    DOIs
    Publication statusPublished - 2018

    Publication series

    NameMethods in Molecular Biology
    Volume1832
    ISSN (Print)1064-3745

    Fingerprint

    Dive into the research topics of 'RChIP-Seq: Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes'. Together they form a unique fingerprint.

    Cite this